• Select the most comprehensive exome and UTR enrichment solution (see Table below).
• Minimize sequencing costs with the superior capture efficiency of our 2.1M overlapping probes.
• Discover more variants compared to all other target enrichment solutions.
• See Nature Biotechnology publication for competitive comparisions

Table 1

Table 1: Coverage comparison of target regions. The SeqCap EZ Exome +UTR Library offers the largest total coverage compared to other exome and UTR products.

Figure 1

Figure 1: SeqCap EZ Exome +UTR Library coverage of raw reads. Coverage comparison of SeqCap EZ Exome +UTR Library and SeqCap EZ Exome Library v3.0 capture sequenced using Illumina 2x76 bp reads. With the addition of 30 Mb UTR content only 1.5 Gb of additional sequencing is required to achieve 90% of bases covered at 10x.

Figure 2

Figure 2: Performance metrics for SeqCap EZ Exome +UTR Library and SeqCap EZ Exome Library v3.0. Research samples were subsampled and sequenced using Illumina 2x76 bp reads and compared for performance. The addition of 30 Mb UTR targets exhibits consistent performance, as shown by the similar results for each metric.

Roche NimbleGen offers SeqCap EZ Library as a solution-based method for target enrichment.

Sequence Capture Protocols

1. Genomic DNA: SeqCap EZ Oligo pool is made against target regions in the genome.
2. Library Preparation: Standard shot-gun sequencing library is made from genomic DNA.
3. Hybridization: The sequencing library is hybridized to the SeqCap EZ Oligo pool.
4. Bead Capture: Streptavidin beads are used to pull down the complex of capture oligos and genomic DNA fragments.
5. Washing: Unbound fragments are removed by washing.
6. Amplification: Enriched fragment pool is amplified by PCR.
7. Enrichment QC: The success of enrichment is measured by qPCR at control loci.
8. Sequencing-Ready DNA: The end product is a sequencing library enriched for target regions, ready for high throughput sequencing.

Download the Annotations

Design & Annotation files were designed for use with the following products:

• SeqCap EZ Exome + UTR Library, 4 Reactions (Catalog No. 06740294001)
• SeqCap EZ Exome + UTR Library, 48 Reactions (Catalog No. 06740308001)

Overview

The SeqCap EZ Exome + UTR Library product is based on the same coding exon sources from SeqCap EZ Human Exome Library v3.0 (described elsewhere in this document) with expanded coverage of 5’- and 3’-untranslated regions (UTRs) from the following sources:

• NCBI Reference Sequence (RefSeq) refGene table from UCSC GRCh37/hg19 March 2012
• Ensembl (GRCh37 v64)

File Descriptions

The folder contains these three files:

• 120430_HG19_ExomeV3_UTR_EZ_HX1_primary_annotated.bed: This file contains primary target intervals along with associated annotation IDs in BED format.
• 120430_HG19_ExomeV3_UTR_EZ_HX1_capture_annotated.bed: his file contains capture target intervals along with associated annotation IDs in BED format. The coordinates listed here correspond to locations where capture probes were actually designed and placed.
  • The BED file can be used to create a custom annotation track for display in the UCSC Genome browser (http://genome.ucsc.edu).

Use SeqCap EZ Hybridization and Wash Kits to perform hybridization and wash steps in sequence capture protocols using NimbleGen SeqCap EZ products. The kits are available in handy 24 and 96 reaction pack sizes.

Description	Catalog Number	Pack Size	Kit Capacity	Compatible Applications	Ordering*
SeqCap EZ Hybridization and Wash Kits	05634261001	1 Kit	24 Reactions	SeqCap EZ Library
	05634253001	1 Kit	96 Reactions
* Availability of products varies from country to country.

Search the SeqCap EZ Library - Literature for more information.