• Customize your own target enrichment discovery tool by adding up to 50 Mb of custom target regions to SeqCap EZ Exome Library v3.0.
• Minimize sequencing costs with the superior capture efficiency of our 2.1M overlapping probes.
• Discover more variants compared to all other target enrichment solutions.
• See Nature Biotechnology publication for competitive comparisions

SeqCap EZ Exome Plus Library exhibits high performance capture efficiency on two custom capture designs (74 Mb and 64.25 Mb), shown below. Similar sequencing metrics illustrate consistent performance with the addition of custom capture targets of varying sizes.

Figure 1

Figure 1: Performance metrics of SeqCap EZ Exome Plus Library. Using Illumina 2x76 bp reads and ~5 Gb sequencing per sample, two SeqCap EZ Exome Plus Libraries (74 Mb and 64.25 Mb custom captures) were compared for performance. The addition of custom capture targets exhibits consistent performance, as shown by the similar results for each metric.

Roche NimbleGen offers SeqCap EZ Library as a solution-based method for target enrichment.

Sequence Capture Protocols

1. Genomic DNA: SeqCap EZ Oligo pool is made against target regions in the genome.
2. Library Preparation: Standard shot-gun sequencing library is made from genomic DNA.
3. Hybridization: The sequencing library is hybridized to the SeqCap EZ Oligo pool.
4. Bead Capture: Streptavidin beads are used to pull down the complex of capture oligos and genomic DNA fragments.
5. Washing: Unbound fragments are removed by washing.
6. Amplification: Enriched fragment pool is amplified by PCR.
7. Enrichment QC: The success of enrichment is measured by qPCR at control loci.
8. Sequencing-Ready DNA: The end product is a sequencing library enriched for target regions, ready for high throughput sequencing.

Design & Annotation files based on the gene sources from SeqCap EZ Human Exome Library v3.0 plus up to 50 Mb of your custom design. Refer to SeqCap EZ Human Exome Library v3.0 Design and Annotation Files for more information.

Use SeqCap EZ Hybridization and Wash Kits to perform hybridization and wash steps in sequence capture protocols using NimbleGen SeqCap EZ products. The kits are available in handy 24 and 96 reaction pack sizes.

Description	Catalog Number	Pack Size	Kit Capacity	Compatible Applications	Ordering*
SeqCap EZ Hybridization and Wash Kits	05634261001	1 Kit	24 Reactions	SeqCap EZ Library
	05634253001	1 Kit	96 Reactions
* Availability of products varies from country to country.

Search the SeqCap EZ Library - Literature for more information.