SeqCap EZ Developer Library provides you the power of up to 2.1 million probes to design your genome specific capture experiment. Built on the same high efficiency target enrichment technology as SeqCap EZ Exome Library, you can now sequence targeted regions of virtually any genome

• Apply our proven capture technology to your research
• Experience high efficiency target enrichment
• Maximize variant discovery while minimizing costs

Figure 1

Figure 1. NimbleGen Sequence Capture design utilizes many more probes than other methods, and this brings important advantages for capture performance. Simple Tiling Designs (Panel A) offer up to 55,000 120mer probes (baits) tiled across the region. NimbleGen Sequence Capture Designs (Panel B) offer up to 2,100,000 50-105mer probes selected for the region using a special algorithm. This dramatic increase in probes (38X) offers the following benefits:
• More attempts for every target base
• Redundancy to reduce risk
• Ability to redisribute probes for better uniformity

Figure 2

Figure 2. Comparing to simple tiling design, probes are relocated in the Sequence Capture design to improve capture uniformity.

Roche NimbleGen offers SeqCap EZ Library as a solution-based method for target enrichment.

Sequence Capture Protocols

1. Genomic DNA: SeqCap EZ Oligo pool is made against target regions in the genome.
2. Library Preparation: Standard shot-gun sequencing library is made from genomic DNA.
3. Hybridization: The sequencing library is hybridized to the SeqCap EZ Oligo pool.
4. Bead Capture: Streptavidin beads are used to pull down the complex of capture oligos and genomic DNA fragments.
5. Washing: Unbound fragments are removed by washing.
6. Amplification: Enriched fragment pool is amplified by PCR.
7. Enrichment QC: The success of enrichment is measured by qPCR at control loci.
8. Sequencing-Ready DNA: The end product is a sequencing library enriched for target regions, ready for high throughput sequencing.

Use SeqCap EZ Hybridization and Wash Kits to perform hybridization and wash steps in sequence capture protocols using NimbleGen SeqCap EZ products. The kits are available in handy 24 and 96 reaction pack sizes.

Description	Catalog Number	Pack Size	Kit Capacity	Compatible Applications	Ordering*
SeqCap EZ Hybridization and Wash Kits	05634261001	1 Kit	24 Reactions	SeqCap EZ Library
	05634253001	1 Kit	96 Reactions
* Availability of products varies from country to country.

SeqCap EZ Developer Reagent serves as a universal reagent to block repetitive regions in the genome of SeqCap EZ Developer Libraries (e.g. plant and other non-human organisms) when species specific Cot-1 DNA is not available.

Search the SeqCap EZ Library - Literature for more information.