Sequence Capture Arrays Quick Guide

Roche NimbleGen offers an optimized workflow for NimbleGen Sequence Capture. The workflow includes arrays, reagents, and instruments necessary to prepare targeted resequencing samples in your laboratory.

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Workflow

  1. Roche NimbleGen certified trainers conduct Sequence Capture customer workshop on-site (3 days) to train researcher on protocols. Contact your local Sales representative for more details.
  2. Roche NimbleGen designs a NimbleGen Sequence Capture Array that targets regions specified by the researcher OR the customer selects a catalog design (2.1M Human Exome). Download the 2.1M Human Exome Annotation Files to see if your gene(s) of interest are on the Exon array.
  3. Researcher orders NimbleGen Sequence Capture Arrays and gets set-up with essential equipment, reagents, and consumables.
  4. Researcher performs Sequence Capture experiment using our validated User’s Guide.
  5. Researcher sequences captured samples on 454 Genome Sequencer FLX (or micro-read technologies not supported by Roche NimbleGen).

Equipment

NimbleGen Hybridization System
Spacing Image 4-bay Hybrization System
NimbleGen Elution System
Spacing Image NimbleGen Elution System
Roche LightCycler® Real-Time PCR System
Spacing Image Roche LightCyler 2.0
  • Determine the success of your Sequence Capture experiment by measuring fold-enrichment across 4 control loci with qPCR
  • Cost-effective method for determination of capture sample quality PRIOR to next-generation sequencing.
  • Find out more...
454 Genome Sequencer FLX System and GS FLX Titanium Series Kits
Spacing Image 454 Genome Sequencer FLX Instrument
  • Captured samples can be fed directly into the 454 GS FLX Titanium Shot-gun library preparation protocol for addition of sequencing adapters.
  • Sequence your captured samples on the 454 GS FLX to generate high-confidence sequencing data using up to ~1,000,000 reads for roughly 500Mb of raw sequence data per picotiter plate.
  • 400bp reads enable haplotyping of 400bp and identification of insertions/deletions from single to tens of base pairs.
  • Find out more...

Reagents

NimbleGen Sequence Capture Array Hybridization and Wash Kits contain the components to perform hybridization and wash steps in sequence capture protocols using NimbleGen Sequence Capture Arrays.

Description Catalog Number Pack Size Kit Capacity Compatible Applications Ordering*
Sequence Capture Array Hybridization and Wash Kit 05853257001 1 Kit 8 Arrays Sequence Capture Arrays Buy Online Button
* Availability of products varies from country to country.

Deliverables

Literature

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