Dual-Color DNA Labeling Kits

Description Catalog Number Pack Size Kit Capacity Compatible Applications Ordering*
Dual-Color DNA Labeling Kit 06370381001 1 Kit 96 Cy3 and 96 Cy5 Labeling Reactions CGH/CNV
ChIP-chip
DNA Methylation		 Contact Us to Purchase
06370250001 1 Kit 24 Cy3 and 24 Cy5 Labeling Reactions Contact Us to Purchase
* Availability of products varies from country to country.
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Applications

NimbleGen Dual-Color DNA Labeling kits are designed for convenient and efficient labeling of DNA samples prior to hybridization to NimbleGen microarrays for CGH/CNV, CGX, ChIP-chip, and DNA Methylation analyses. These kits are based on Cy3- or Cy5 random priming, and use a high concentration of exo- Klenow, lacking both proofreading (3’→ 5’) and nick translation(5’→ 3’) nuclease activities to incorporate dNTPs while replicating the DNA.

The new labeling kits offer several content and protocol improvements over the older kit: optimized Cy3- and Cy5 random primers, and a simplified “Stop Solution” (used for terminating the labeling reaction) which is now added in a single step. This optimized and streamlined protocol leads to more robust labeling yields, improved signal to noise ratios (CGH/CNV), and will enable you to achieve a higher-throughput yet economical sample analysis. When used in combination with the NimbleGen Hybridization and Wash Buffer kits, the DNA Labeling kits ensure high signal-to-noise ratios and highly sensitive signals for NimbleGen microarray applications.

192 Reaction Kit (Catalog Number: 06370381001)

Dual-Color DNA Labeling Kit (96 Cy3 and 96 Cy5 Labeling Reactions)

This kit provides sufficient reagents for 96 Cy3 labeling reactions and 96 Cy5 labeling reactions. For all applications and platforms available one labeling reaction (of Cy3 and Cy5) is sufficient per hybridization, starting with 500ng DNA (CGH/CNV, CGX) or 1ug DNA (ChIP-chip and DNA Methylation).

Kit Contents

Component Vial Number Cap Color Quantity
PCR Grade Water 1 Clear 2 x 7.25ml
Random Primer Buffer 2 Clear 2 x 4.25ml
Cy3-Random Nonamers 3 Green 4 x 25 O.D.
Cy5-Random Nonamers 4 Red 4 x 25 O.D
Klenow Fragment (3'->5' exo-) 50U/µl 5 Yellow 2 x 200 μl
dNTP Mix (10mM each dNTP) 6 Purple 2 x 1.0ml
Stop Solution 7 Clear 2 x 2.1ml

48 Reaction Kit (Catalog Number: 06370250001)

Dual-Color DNA Labeling Kit (24 Cy3 and 24 Cy5 Labeling Reactions)

This kit provides sufficient reagents for 24 Cy3 labeling reactions and 24 Cy5 labeling reactions. For all applications and platforms available one labeling reaction (of Cy3 and Cy5) is sufficient per hybridization, starting with 500ng DNA (CGH/CNV, CGX) or 1ug DNA (ChIP-chip and DNA Methylation).

Kit Contents

Component Vial Number Cap Color Quantity
PCR Grade Water 1 Clear 2 x 1.85ml
Random Primer Buffer 2 Blue 2 x 1.1ml
Cy3-Random Nonamers 3 Green 1 x 25 O.D.
Cy5-Random Nonamers 4 Red 1 x 25 O.D
Klenow Fragment (3'->5' exo-) 50U/µl 5 Yellow 2 x 50 μl
dNTP Mix (10mM each dNTP) 6 Purple 2 x 250 μl
Stop Solution 7 Brown 2 x 550 μl

Literature

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Technical Notes

Labeling Protocols

Labeling protocols for CGH, CGH/LOH, CGX, ChIP-chip, and DNA Methylation arrays can be found in the corresponding User's Guides below:

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