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NimbleGen Kits & Consumables 
Figure 1. Workflow for CGH, ChIP-chip, DNA Methylation and Gene Expression arrays. For Sequence Capture workflow, visit the Sequence Capture page. Roche NimbleGen provides an integrated solution for your genomics research needs, including microarrays, kits, consumables, instruments, software, and training. Together with the robust protocols provided for every step of your microarray analysis, from sample labeling to data analysis (Figure 1), these optimized reagents will enable you to conveniently and efficiently achieve the same high quality data in your own laboratory as that provided by the NimbleGen microarray service lab. For our new Sequence Capture for Delivery product, we offer two kits for processing Sequence Capture arrays in your own lab. These kits allow efficient and convenient hybridization, washing, and elution of target DNA fragments for sequencing. Ordering Information
 
The NimbleGen Dual-Color (shown above) and One-Color DNA Labeling kits are designed for convenient and efficient labeling of DNA samples prior to hybridization to NimbleGen microarrays for CGH (Dual-Color), ChIP-chip (Dual-Color), DNA Methylation (Dual-Color), and Gene Expression (One-Color) analyses. These kits are based on Cy3- or Cy5-random priming and use a high concentration of exo- Klenow, lacking 3’ -> 5’ exonuclease activity, to incorporate dNTPs while replicating the DNA. This optimized protocol leads to high sample labeling yields and robust microarray performance. When used in combination with the NimbleGen Hybridization and Wash Buffer kits, the DNA Labeling kits ensure high signal-to-noise ratios and highly sensitive signals for NimbleGen microarray applications.
NimbleGen Array Processing Accessories contain the necessary labware to use when washing NimbleGen arrays after hybridization. The accessories are designed specifically for NimbleGen CGH, ChIP-chip, DNA Methylation, and Gene Expression microarray applications.
Array Re-Use Kits
Mixers
NimbleGen mixers, which you adhere directly to microarray slides before sample loading and hybridization, are a key component of the NimbleGen Hybridization System. The mixers allow for low hybridization volume, leading to greater sensitivity and reliable results while economizing on sample requirements. They fit NimbleGen arrays precisely and are optimized for NimbleGen array dimensions, ensuring uniform signal and high sensitivity with minimal sample volume.
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