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NimbleGen Kits & Consumables Hybridization Kits Sample Tracking Controls Wash Buffer Kit Array Processing Accessories

Roche NimbleGen provides an integrated solution for your genomics and epigenomics research needs, including NimbleGen microarrays, instrumentation, software, kits and consumables, and training. We supply robust, easy-to-use protocols for all product applications, enabling you to process microarrays with quality results.

Our consumables include NimbleGen mixers, which you adhere to the microarray before sample loading to ensure uniform signal and high sensitivity with minimal sample volume. Our easy-to-use microarray hybridization and wash reagents are optimized for maximum sensitivity and signal-to-noise ratios for all catalog NimbleGen microarray applications and formats. Sample tracking controls facilitate confirmation of sample identity on multiplex arrays.

Note: On May 5th, 2008, Roche NimbleGen introduced new kits to better support the hybridization and processing requirements of NimbleGen high-density (HD2, 2.1million probe) and multiplex microarrays, in addition to 385K microarrays. These newly configured Hybridization Kits and Wash Buffer Kit contain the same formulations of highly optimized reagents as the discontinued kits. Read more...

Ordering Information
Catalog Number Name Compatible Applications Kit
Capacity
01002 NimbleGen
Hybridization Kit		 CGH
ChIP-chip
DNA Methylation
Gene Expression		 See Below
Format* Number of Hybridizations Number of Slides
385K 100 100
385K 4-Plex (4x72K) 160 40
HD2 40 40
HD2 12-plex (12x135K) 120 10
* Not all applications are available in all formats.
01003 NimbleGen
Hybridization Kit, LS		 CGH
ChIP-chip
DNA Methylation
Gene Expression		 See Below
Format* Number of Hybridizations Number of Slides
385K 300 300
385K 4-Plex (4x72K) 480 120
HD2 120 120
HD2 12-plex (12x135K) 480 40
* Not all applications are available in all formats.
00994 NimbleGen
Sample Tracking Control Kit		 CGH
Gene Expression		 See Below
Format Number of Hybridizations Number of Slides
385K 4-Plex (4x72K) 480 120
HD2 12-Plex (12x135K) 480 40
01004 NimbleGen
Wash Buffer Kit		 CGH
ChIP-chip
DNA Methylation
Gene Expression		 See Below
Format Number of Washes Number of Slides
All 10 10 - 40
01011 NimbleGen
Array Processing Accessories		 CGH
ChIP-chip
DNA Methylation
Gene Expression		 Unlimited
NimbleGen Hybridization Kits

NimbleGen Hybridization Kit and NimbleGen Hybridization Kit, LS contain the necessary components for sample hybridization to NimbleGen arrays. The kits are designed specifically for NimbleGen CGH, ChIP-chip, DNA Methylation, and Gene Expression microarray applications.

The NimbleGen Hybridization kits incorporate a set of highly optimized hybridization reagents to ensure high signal-to-noise ratios and high sensitivity hybridization for all NimbleGen microarray applications. The proprietary hybridization buffer was formulated to maximize stringent signal strength without sacrificing specificity. The kits include a control Alignment Oligo that hybridizes to grid alignment features included on all NimbleGen arrays.

The number of hybridizations you can perform using a NimbleGen Hybridization Kit depends on the format of the microarrays (single or multiplex) to which samples are being hybridized.

Catalog Number Name Kit
Capacity		 Kit
Components
01002 NimbleGen Hybridization Kit See Below See Below
Format* Number of Hybridizations Number of Slides
385K 100 100
385K 4-Plex (4x72K) 160 40
HD2 40 40
HD2 12-plex (12x135K) 120 10
* Not all applications are available in all formats.
Component Storage Temperature 1 Quantity Volume per Tube
2X Hybridization Buffer 4°C 1 1.20ml
Hybridization Component A 4°C 1 0.5ml
Alignment Oligo 2 -20°C 3 1 55µl
1 Components in the kit require storage at differing temperatures. Upon receipt of the kit, remove the components and store them at the recommended storage temperatures noted in the table above.
2 The Alignment Oligo is a mixture of Cy3- and Cy5-labeled 48mer oligonucleotides that hybridize to alignment features on NimbleGen arrays. It is required for proper extraction of array data from the scanned image.
3 The Alignment Oligo is labeled with Cy™ dyes and is sensitive to photobleaching and freeze-thawing. After thawing stock tubes for the first time, aliquot the Alignment Oligo into single-use volumes and freeze at -20°C. Protect tubes from light.
01003 NimbleGen Hybridization Kit, LS See Below See Below
Format* Number of Hybridizations Number of Slides
385K 300 300
385K 4-Plex (4x72K) 480 120
HD2 120 120
HD2 12-plex (12x135K) 480 40
* Not all applications are available in all formats.
Component Storage Temperature 1 Quantity Volume per Tube
2X Hybridization Buffer 4°C 3 1.20ml
Hybridization Component A 4°C 3 0.5ml
Alignment Oligo 2 -20°C 3 3 55µl
1 Components in the kit require storage at differing temperatures. Upon receipt of the kit, remove the components and store them at the recommended storage temperatures noted in the table above.
2 The Alignment Oligo is a mixture of Cy3- and Cy5-labeled 48mer oligonucleotides that hybridize to alignment features on NimbleGen arrays. It is required for proper extraction of array data from the scanned image.
3 The Alignment Oligo is labeled with Cy™ dyes and is sensitive to photobleaching and freeze-thawing. After thawing stock tubes for the first time, aliquot the Alignment Oligo into single-use volumes and freeze at -20°C. Protect tubes from light.
NimbleGen Sample Tracking Control Kit

The NimbleGen Sample Tracking Control Kit contains 12 Sample Tracking Controls (STCs). A unique STC is added to each sample before hybridization to a multiplex array, enabling you to confirm that the correct sample was hybridized to each array. The kit is designed specifically for NimbleGen CGH and Gene Expression multiplex microarray applications.

You can perform 480 hybridizations to 385K 4-plex arrays with the components in the NimbleGen Sample Tracking Control kit.

Catalog Number Name Kit
Capacity		 Kit
Components
00994 NimbleGen Sample Tracking Control Kit See Below See Below
Format Number of Hybridizations Number of Slides
385K 4-Plex (4x72K) 480 120
HD2 12-Plex (12x135K) 480 40
Component 1 Part Number 2 Storage Temperature 3 Quantity Volume per Tube
Sample Tracking Control 1
Sample Tracking Control 3
Sample Tracking Control 5
Sample Tracking Control 7
Sample Tracking Control 9
Sample Tracking Control 11
Sample Tracking Control 13
Sample Tracking Control 15
Sample Tracking Control 16
Sample Tracking Control 17
Sample Tracking Control 18
Sample Tracking Control 20		 01012
01014
01016
01018
01020
01022
01024
01026
01027
01028
01029
01031		 -20°C 1 of each unique STC 140µl
1 Each STC is a Cy3-labeled 48mer oligonucleotide that hybridizes to sample tracking control probes on NimbleGen arrays.
2 Part numbers are provided for reference only. STCs cannot be purchased individually.
3 The STCs are labeled with Cy3™ dye and are sensitive to photobleaching and freeze-thawing. After thawing stock tubes for the first time, aliquot the STCs into single-use volumes and freeze at -20°C. Protect tubes from light.
NimbleGen Wash Buffer Kit

The NimbleGen Wash Buffer kit contains optimized buffers to ensure efficient washing of NimbleGen arrays after hybridization. The kit is designed specifically for NimbleGen CGH, ChIP-chip, DNA Methylation, and Gene Expression microarray applications.

You can perform 10 washes, processing up to 4 slides per wash, with the components in the NimbleGen Wash Buffer kit.

Catalog Number Name Kit
Capacity		 Kit
Components
01004 NimbleGen Wash Buffer Kit See Below See Below
Format Number of Washes Number of Slides
All 10 10 - 40
Component Storage Temperature 1 Quantity Volume per Tube
10X Wash Buffer I Room Temperature 1 500ml
10X Wash Buffer II Room Temperature 1 250ml
10X Wash Buffer III Room Temperature 1 250ml
1M DDT -20°C 1 1ml
1 Components in the kits require storage at differing temperatures. Upon receipt of the NimbleGen Wash Buffer kit, remove components and store them at the recommended storage temperatures.
NimbleGen Array Processing Accessories

NimbleGen Array Processing Accessories contain the necessary labware to use when washing NimbleGen arrays after hybridization. The accessories are designed specifically for NimbleGen CGH, ChIP-chip, DNA Methylation, and Gene Expression microarray applications.

Catalog Number Name Kit
Capacity		 Kit
Components
01011 NimbleGen Array Processing Accessories Unlimited See Below
Component Quantity  
Slide Rack 1
Wash Tank 4
Slide Container 1 8 (2 packages of 4)
1 Washes one slide at a time.
NimbleGen Array Re-Use Kits

These kits provide all the reagents required to gently but thoroughly remove previously hybridized sample from NimbleGen arrays without adversely affecting probe performance through additional rounds of hybridization. NimbleGen arrays may be used multiple times depending on the application and sample quality.

Note: NimbleGen does not guarantee array reuse. Array reuse is not recommended for Gene Expression arrays.

Catalog Number Name Number of Slides Components
KIT001-2 NimbleGen Array Reuse Kit 40 40 See Below
Component Quantity
Array Reuse Solution 2 x 500ml
Processing Tanks 2
Array Processing Racks 1
Slide Processing Containers 4
KIT004-2 NimbleGen Array Reuse Kit Refill 40 40 See Below
Component Quantity
Array Reuse Solution 2 x 500ml
KIT001-3 NimbleGen Array Reuse Kit 100 100 See Below
Component Quantity
Array Reuse Solution 5 x 500ml
Processing Tanks 2
Array Processing Racks 1
Slide Processing Containers 4
KIT004-3 NimbleGen Array Reuse Kit Refill 100 100 See Below
Component Quantity
Array Reuse Solution 5 x 500ml
NimbleGen Mixers

NimbleGen mixers, which you adhere directly to microarray slides before sample loading and hybridization, are a key component of the NimbleGen Hybridization System. The mixers allow for low hybridization volume, leading to greater sensitivity and reliable results while economizing on sample requirements. They fit NimbleGen arrays precisely and are optimized for NimbleGen array dimensions, ensuring uniform signal and high sensitivity with minimal sample volume. Mixers are supplied with your NimbleGen arrays or can be purchased separately.

NimbleGen Mixers
Name: NimbleGen X1 Mixer
Compatible Arrays: 385K
Sample Loading Volume: 16µl
Catalog Number Quantity
00798 5 Mixers
00799 25 Mixers
00800 100 Mixers
Name: NimbleGen X4 Mixer
Compatible Arrays: 4x72K
Sample Loading Volume: 8µl
Catalog Number Quantity
00804 5 Mixers
00805 25 Mixers
00806 100 Mixers
Name: NimbleGen HX1 Mixer
Compatible Arrays: HD2
Sample Loading Volume: 41µl
Catalog Number Quantity
00810 5 Mixers
00811 25 Mixers
00812 100 Mixers
Name: NimbleGen HX12 Mixer
Compatible Arrays: 12x135K
Sample Loading Volume: 6µl
Catalog Number Quantity
00819 5 Mixers
00820 25 Mixers
00821 100 Mixers
Literature

For a complete listing of literature covering all Roche NimbleGen products and services please visit our literature page.

  

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