MS 200 Microarray Scanner

MS 200 Microarray Scanner Image MS 200 Video Button
Description Catalog Number Pack Size Ordering*
MS 200 Microarray Scanner 05394341001 1 Instrument Buy Online Button
MS 200 Microarray Scanner Slide Magazine 05471478001 1 Accessory Buy Online Button
* Availability of products varies from country to country.


Advantages

  • 2µm pixel resolution for optimal scanning of high-density NimbleGen DNA microarrays
  • Autoloader for automated batch processing of up to 48 slides for 24-hour unattended operation
  • High signal-to-noise for high quality data and confident results
  • Integrated barcode reader for sample identification and tracking
  • Advanced dynamic autofocus and auto gain for a clear, bright scan every time
  • Internal QC reference slide for easy calibration; scan with confidence every time
  • Isolated slide magazine and carbon-filtered airflow to reduce degradation of fluorescent dyes
  • Upgradeable to ensure compatibility with high-density NimbleGen arrays of today and ultra-high density arrays of tomorrow

Applications

Figure 1

Figure 1: Images of a NimbleGen Array. Comparison of a NimbleGen array scanned at 2μm and 5μm resolution. The increased resolution at 2μm scanning enables high data quality and more confident results from high-density NimbleGen microarrays with the flexibility to scan other array formats.

Figure 2

Figure 2: Signal-to-Noise Performance Data. The MS 200 at 2μm resolution provides ~1.4x higher signal-to-noise compared with 5μm resolution scans. This improved resolution provides higher data quality with the reduced noise for more confident results.


Figure 3

Figure 3: CGH Analysis of a Burkitt Lymphoma Sample. Chromosomal copy number gains and losses were identified in a Burkitt Lymphoma research sample referenced against normal genomic DNA using a NimbleGen Human CGH 3x720K Whole-Genome Tiling v3.0 array. A large deletion (from ~8kb – 43Mb) and amplification (from ~47Mb – 110Mb) in chromosome 8, detected by scanning at either 2μm or 5μm resolution, as indicated. The red and blue bars indicate a signal-to-noise ratio of > 6 (red bars) or less than 6 (blue bars). Note that the 2μm scan indicates better signal-to-noise ratios (indicated by more red bars), especially in the region of copy number amplification.

Specifications

Pixel resolution 2μm, 5μm, 10μm, 20μm, 40μm
Dynamic range 105 ( 16-bit data format)
Recommended dyes Cyanine 3 (Cy3) and Cyanine 5 (Cy5)
Uniformity <4% CV global non-uniformity based on 22 x 60mm scan area
Limit of detection 0.0025 Cy3; 0.005 Cy5 (chromophores/μm2)
Autofocus Auto-adjusts for optimal focus position and angle to ensure features stay in focus while scanning
Light sources Two solid-state lasers (532nm and 635nm)
Slide magazine Holds up to 48 slides for walkaway automation
Slide scanning speed 2-color simultaneous data acquisition in 28 minutes for a 2.1M array at 2μm resolution with auto gain on
Auto gain adjustment 1-1000%; channels balanced
Scan window maximum 22 x 75mm
Pixel placement error <2 pixel shift in spatial position per 7,200 pixels scanned
Supported glass specifications 25 x 75mm (1 x 3in) glass slides - Agilent and NimbleGen glass slide dimensions

Detailed dimensions:
Width: 24.6 - 26.0mm
Length: 75.0 - 76.5mm
Thickness: 0.8 - 1.2mm
Supports non-mirrored glass slides
Barcode reader Reads Code 39 and Code 128 barcode formats
PC operating system Microsoft Windows Vista Business (64-bit)
Outer dimensions Width: 60.0cm (23.6in)
Height: 46.6cm (18.5in)
Depth: 61.8cm (24.4in)
Weight Approximately 38kg (84.0lb)
Power supply Auto sensing: 100 - 120V / 220 - 240V, 50/60Hz

What's Included?

The following are included with the purchase of an MS 200:
Catalog Number Name
05394341001 1 MS 200 Microarray Scanner
05471478001 1 MS 200 Slide Magazine
05394325001 1 MS Control Unit
05394333001 1 MS 200 Software
05394309001 1 MS 200 Operator’s Manual
05933315001 3 individual user licenses of NimbleScan software
05225051001 3 individual user licenses of SignalMap software

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