NimbleGen Masthead
 
spacer Product Images

Gene Expression - Custom Designs


Roche NimbleGen’s proprietary array manufacturing technology allows fast and cost-effective synthesis of custom-designed arrays. Custom-designed arrays may be ordered for delivery (for hybridization in your own lab) for any organism.

Custom designs may also be ordered for full service analysis if they meet the design principles described below. These principles will ensure best performance using the standard NimbleGen array processing protocols.

Custom Design Principles for Full-Service Workflow

  • Transcriptome GC content is between 35 and 60%
  • Probe selection is ORF-based with 3’-bias
  • Probe length is 60-mer for eukaryotes and 45-60-mer for prokaryotes
  • Transcriptome size allows majority of transcript to have ≥ 3 probes per target

Please contact Roche NimbleGen Sales for a quotation or contact NimbleGen Technical Support with any questions about gene expression custom arrays.

Array Specs
Array Format 385K 4x72K 12x135K
Number of Arrays per Slide 1 4 12
Total Number of Probes 385,000 4 x
72,000
12 x
135,000
Probe Length 60mer 60mer 60mer
Number of Probes per Target* ≥ 3 ≥ 3 ≥ 3
Number of Replicate Probe Sets per Array* Varies Varies Varies
Feature Size 16 x 16μm 16 x 16μm 13 x 13μm
Array Size 17.4 x
13mm
7.8 x
5.7mm
8.9 x
6.5mm
Slide Size 1 x 3 in. (25 x 76 mm)
Recommended Storage Desiccated at room temperature.
* Depending on transcriptome size.
Populus

These whole-genome Populus arrays were designed in collaboration with Dr. Steve DiFazio at West Virginia University (http://www.as.wvu.edu/~sdifazio/). They target all predicted genes in the Populus nuclear and organellar genomes.

Catalog Number Design Name Description
Genes
Probes/ Target
Replicates
A7412-00-01 080401 Populus 385K EXP Poplar 385K Array
52,225
7
1
A7411-00-01 080925 Populus 135K EXP Poplar 12x135K Array
48,276
3
1
 

CONTACT US

ONLINE TRAINING

NEW RESEARCH

WEBINARS

LITERATURE