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Gene Expression Microarrays and
Services

NimbleGen Systems provides a unique combination of high-density arrays, long-oligo probes, and flexible design capability that together provide superior results for advanced gene expression analysis. NimbleGen’s technology enables accurate, sensitive, and specific interrogation of genome-wide expression for any sequenced and annotated genome. Researchers also can tile through a genome at any desired spacing to discover genome-wide expression activity in both coding and noncoding regions and map transcripts with unparalleled accuracy. NimbleGen’s microarrays and services for both prokaryotic and eukaryotic genomes are the gene expression platform of choice as evidenced by a growing list of scientific publications.

Advantages and Applications

Advantages

More Probes per Transcript

The ultra-high density of NimbleGen’s arrays (385,000 features currently, 2.1 million available in 2008) allows every gene to be represented by multiple, unique probes. The averaging of the signal from multiple probes provides improved statistical confidence, reducing the impact of inconsistent probe behavior and improving the signal-to-noise ratio compared to platforms that offer fewer probes per gene.

Long Oligos Enhance Accuracy

NimbleGen has an unmatched ability to synthesize high-density arrays of long oligos. For gene expression analysis, long oligos (60mer) provide superior signal-to-noise ratio, increased sensitivity, specificity, and discrimination, particularly in complex eukaryotic genomes. Another significant advantage: long probes eliminate the need for mismatch probes, effectively doubling array capacity.

Multiplex Options: Cost-Effective, High-Throughput Analysis

NimbleGen multiplex arrays enable you to analyze samples in parallel. We offer a 4-plex design with 72,000 features per array. Hybridize four independent samples or run replicate samples on a single slide and average the data for increased statistical confidence. Learn more...

Up-to-date Microarray Design

NimbleGen's digital microarray manufacturing process is fast and inexpensive, allowing you to get any sequenced genome on an array rapidly. All designs are built from the latest published genomes to ensure your experiment is current.

Great Chip-to-Chip Reproducibility

NimbleGen arrays are manufactured to exacting standards and undergo rigorous quality control to ensure superior chip-to-chip data reproducibility. In replicate hybridization experiments, inter-chip r2 values routinely exceed 0.98.

Applications

An increasing number of scientists are taking advantage of the inherent flexibility, improved sensitivity, and discrimination provided by NimbleGen's gene expression microarray technology for numerous applications as evidenced by the number of peer-reviewed publications citing this platform:

Differential Expression Analysis

  • Study of gene expression related to multiple signal pathways in tissues and cells
  • Identification of factors involved in plant tissue differentiation
  • Discovery of regulatory targets for an orphan two-component signal regulator and virulence factor in Streptococcus pneumoniae
  • Assessment of differential gene expression of closely related protozoan parasites

Tiling

  • Identification and mapping of the non-coding RNAs in human fibroblasts and yeast
  • Identification of novel transcripts in rice or Populus trichocarpa
  • Analysis of global gene expression in Homo sapiens, Drosophila melanogaster, Chlamydomonas reinhardtii, Arabidopsis thaliana, and rice

Allele-Specific Expression

  • Analyze allele-specific expression in the cotton genome
Gene Expression Array Designs

Our extensive catalog of gene expression designs contains hundreds of prokaryotic organisms, the major model organisms, and a growing list of eukaryotic organisms. NimbleGen is advancing the current array technology not only by dramatically increasing density and information content per array, but also by introducing multiplex formats that allow multiple samples to be hybridized to the slide independently. You can choose to hybridize your samples and analyze the data in your lab using our protocols and reagents. Alternatively, send your samples to us for processing in our full-service laboratory, results guaranteed.

Eukaryotic Gene Expression Arrays

NimbleGen’s broad range of eukaryotic arrays represent the latest builds for human and most model genomes, including rat, mouse, zebrafish, Drosophila, C. elegans, yeast, and more. Many designs are available in both single and multiplex design formats, allowing for both genome-wide probe coverage and high-throughput analysis.

Prokaryotic Gene Expression Arrays

NimbleGen offers the most comprehensive set of prokaryotic gene expression designs in the industry, covering hundreds of Class I, II, and III organisms and growing as new genomes become available. Because each expression microarray contains up to 385,000 features per array on a 1-plex format and 72,000 features per array on a 4-plex format, there is sufficient probe capacity to tailor an existing array design to address specific research questions. Consider including:

  • Relevant plasmids
  • Probes from intergenic regions
  • Probes from multiple organisms
  • Your own custom controls

Our high-density arrays allow for every gene in a prokaryotic genome to be represented by 7 - 25 replicate, gene-specific probes, depending on the size of the genome, therefore increasing the statistical confidence of the experiment.

With the new prokaryotic gene expression 4-plex arrays for service and delivery, customers now have the convenience of hybridizing four individual samples on each slide, thus increasing efficiency and reducing cost. Each array contains 72,000 features, allowing up to 17 probes per genome, depending on the genome size.

Custom-designed multiplex arrays for delivery may also be ordered for any organism with high-quality sequence and annotation information. Please contact NimbleGen Sales for a quotation or contact NimbleGen Technical Support with any questions about gene expression custom arrays.

NimbleGen Multiplex Arrays

NimbleGen is pleased to announce the availability of 4-plex prokaryotic gene expression arrays for service and for analysis in your own laboratory. As with our existing 4-plex eukaryotic gene expression arrays, you have the ability to analyze multiple samples in replicate, high-density arrays on a single slide. NimbleGen Multiplex Arrays offer an extremely cost-effective approach to analyzing gene expression, without compromising information content or data quality. Our use of long oligo (60mer) probes ensures high specificity, sensitivity, and reproducibility.

Four individual samples may be hybridized and analyzed simultaneously on each Multiplex Array. Whole transcriptome sets are available in multiplex format for many prokaryotic species and eukaryotic species including human and a broad range of model organisms: mouse, rat, fruit fly, zebrafish, A. thaliana, C. elegans, S. cerevisiae, and S. pombe. Custom-designed multiplex arrays may also be ordered for other organisms with high-quality sequence and annotation information.

Advantages of NimbleGen Multiplex Gene Expression Analysis

  • Cost-effective solution for testing multiple samples
    Our multiplex arrays are sufficiently cost-effective that you can now analyze statistically meaningful populations for alterations in expression levels of annotated genes. Each 4-plex array can be used to analyze four independent samples, or you may choose to analyze three test samples and a common control on each set of arrays.
  • High information content: Multiple probes per transcript enhances confidence in results
    NimbleGen high density arrays contain probes targeting multiple regions of every transcript, thereby providing more information than competitive platforms with either single or multiple arrays, increasing confidence in your results.
  • Long oligonucleotide probes enhance the accuracy of your analysis
    Our long probes enable you to more precisely measure transcript levels, whether increased or reduced. Extensive studies have shown that long probes provide increased sensitivity, specificity and reproducibility.
  • Robust protocols
    We will provide you with validated protocols for use of arrays in your own lab, or you may provide us with samples and we will perform the full analysis on a service basis.
  • ArrayStar™ v2.0 expression analysis software
    Developed in collaboration with DNASTAR, this software enables easy and effective analysis of gene expression levels on a genome-wide basis.
  • Comprehensive technical support available toll-free to help ensure your success
4-Plex Gene Expression Array Specifications
Probe length 60mer (eukaryote), 45 – 60mer (prokaryote)
Probe/transcript 2 – 20 (depending on organism)
Total features 72,000 per array
Feature Size 16μm x 16μm
Array size (7.8mm x 5.7mm) x 4 arrays
Slide size 1" x 3" (25 x 76mm) glass

Gene Expression 4-Plex Prokaryotic and Eukaryotic Array Delivery

Customers can purchase catalog arrays or custom arrays from NimbleGen and perform the array experiments in their own laboratories or core facilities. NimbleGen arrays are synthesized on standard-sized glass microscope slides compatible with many microarray scanners. NimbleGen offers a line of hybridization instruments, kits and consumables, and provides a complete user’s guide to support customers with sample labeling, array hybridization, scanning, data extraction, and data analysis. Please contact NimbleGen for a list of required equipment and reagents. NimbleGen also provides NimbleScan™ and ArrayStar™ v2.0 (30-day demonstration version) software tools which enable the same data analysis and visualization to be performed in your laboratory as in NimbleGen full service. NimbleGen also offers a training program to get you up and running with NimbleGen arrays quickly.

Gene Expression 4-Plex Prokaryotic and Eukaryotic Full Service

Customers can select catalog arrays or work with NimbleGen bioinformatics scientists to create custom arrays that best suit your experimental goals. Customers send samples (RNA or cDNA) to NimbleGen, and we perform the sample labeling, array hybridization, scanning, data extraction, and preliminary data analysis. We will provide the customer with raw and normalized expression data, together with ArrayStar™ v2.0 (30-day demonstration version) software for further data analysis.

Service and Delivery Options

Gene Expression Array Service

NimbleGen's gene expression microarray service consists of the following steps:

  1. Customer selects catalog array design, or works with NimbleGen bioinformatics scientists to create a custom array.
  2. Customer supplies RNA or cDNA. To ensure sufficient sample for quality control analysis as well as optimum hybridization, we require the following amounts of total RNA, mRNA, or cDNA:
      Total RNA* mRNA* cDNA
    Eukaryote 20μg 5μg 2.5μg
    Class I Prokaryotes 20μg - 2.5μg
    Class II and III Prokaryotes 30μg - 5μg
    *Please note that the NimbleGen procedure for sample processing does not include RNA amplification.
  3. NimbleGen performs the expression hybridization, scans the array, extracts the data, and performs a preliminary data analysis.
  4. NimbleGen delivers the following data and software to customer:
    • Microarray Design Files
    • Probe Sequences
    • Gene Descriptions
    • Raw Data
    • Normalized Data
    • ArrayStar v2.0 expression analysis software.

Gene Expression Array Delivery

Customers can purchase catalog arrays or custom arrays from NimbleGen and perform the array experiments at their own laboratories or core facilities. NimbleGen arrays are synthesized on standard-sized glass microscope slides, compatible with many microarray scanners. NimbleGen offers a line of hybridization instruments, kits and consumables as well as provides a complete user’s guide to support customers with sample labeling, array hybridization, scanning, data extraction and analysis. Please contact NimbleGen for a list of required equipment and reagents. NimbleGen also provides NimbleScan™ and ArrayStar™ v2.0 (30-day demonstration version) software tools which enable the same data analysis and visualization to be performed in your laboratory as in NimbleGen full service. Customers who are working with 4-plex arrays should download the free NimbleScan2 Sample Tracking PlugIn. NimbleGen also offers a training program to get you up and running with NimbleGen arrays quickly.

Array Specifications
Probe length 60mer
Probe/transcript 6 - 25 (1-plex format)
2 - 20 (4-plex format)
Total features 385,000 per array (1-plex format)
4 x 72,000 per array (4-plex format)
Feature Size 16μm x 16μm
Array size 17.4mm x 13mm (1-plex format)
7.8mm x 5.7mm (4-plex format)
Slide size 1" x 3" (25 x 76mm) glass
FAQ
Multiplex
What is the advantage of the NimbleGen 4-plex array platform? The NimbleGen 4-plex array platform allows simultaneous hybridization of 4 samples on a single slide. You can conveniently run either replicates of the same sample or multiple samples on the same slide, thus reducing cost.
How many features are on each array of the NimbleGen 4-plex format? Each array on the NimbleGen 4-plex slide has 72,000 features.
Do you offer multiple probes per transcript in the NimbleGen 4-plex format? Yes, each transcript is interrogated by 3 or more probes, depending on the complexity of the organism. Please see our catalog for more details for each organism.
What kind of controls do you include on each array to ensure accurate sample loading and to confirm the integrity of the experiment? Each array contains probes for Sample Tracking Controls (STCs) that are used for the following purposes:
a) To identify the samples after hybridization; each labeled cDNA sample is spiked with a unique Sample Tracking control before loading.
b)To ensure that there is no sample mix up between arrays.
Do all arrays on a NimbleGen 4-plex slide have the same design? Yes. Currently all arrays on a gene expression multiplex array have identical designs.
Array Design
Do you include microRNAs or non-coding RNA in your catalog designs for expression? Currently, no microRNA or non-coding RNA regions are used for NimbleGen gene expression array designs.
What is the probe length in gene expression arrays? NimbleGen expression arrays contain 60-mer probes.
Do you offer catalog and custom array designs? Yes, we offer both catalog and custom array designs for differential expression analysis. Please check our website for the current catalog. If you do not see your organism, please contact the Account Manager for your region to discuss a custom design.
Are your gene expression probes designed with a 3' bias? Yes, probes are designed with some 3' bias. Our expression arrays are designed by first determining the transcript terminator as annotated in NCBI. We then take 1500bp upstream of that location and design our probes within that region.
Which strand is represented on the array? The sense strand is represented on our arrays.
Can NimbleGen design a custom expression tiling array for my specific research needs? Yes, NimbleGen can design and deliver to you custom expression tiling microarrays with probe spacing and probe length that best fits your experimental goals. Please contact the Account Manager for your region to discuss a custom design.
Are NimbleGen designs up-to-date with the latest genome builds? Yes, NimbleGen technology allows the rapid production of new array designs based on the latest genome builds.
Are designs based on earlier genome builds available? Yes, please contact the Account Manager for your region.
Sample Preparation
What is the recommended protocol for isolating RNA? We recommend the RNeasy Mini Kit (Qiagen, Cat. No. 74104) or the TRIzol Reagent (Invitrogen, Cat. No. 15596-026)). If using TRIzol-isolated RNA for cDNA synthesis, it is beneficial to perform a secondary cleanup step using RNeasy Mini Kit. Immediately after the ethanol precipitation step in the TRIzol procedure, proceed with the RNeasy Mini Kit according to the manufacturers recommendations.
Can I use a different RNA isolation method than recommended? Any RNA isolation method that provides RNA which meets the purity and integrity criteria outlined by NimbleGen can be used.
How do you assess the quality of RNA and cDNA samples? Pure and intact RNA and cDNA should have A260/A280 and A260/A230 ratios of at least 1.8. Additionally, they should appear intact when analyzed by gel electrophoresis or using a Bioanalyzer (Agilent).
How much RNA or cDNA do you need for gene expression analysis? This depends on whether you are sending the samples to NimbleGen for full service or if you are doing the experiment in your lab, and whether your sample is from eukaryotes or prokaryotes. When performing experiments in your lab using NimbleGen-recommended protocol, 10 μg total RNA, 1 μg mRNA, or 1 μg cDNA is required. For service, the amounts required are listed in the table below.
 
  Total RNA* mRNA* cDNA
Eukaryote 20μg 10μg 4μg
Class I Prokaryote 20μg - 4μg
Class II and III Prokaryotes 30μg - 8μg
*Please note that the NimbleGen procedure for sample processing does not include RNA amplification.
What is the expected cDNA yield from 10μg total eukaryotic RNA? Using our standard protocol, we routinely get at least 5μg cDNA from 10μg total RNA.
If I do not have 10μg of RNA, what method for RNA amplification can I use in my own lab? We have successfully used the MessageAmp II aRNA Amplification kit (Ambion, Cat. No. 1751) for RNA amplification of two human reference samples: Universal Human Reference RNA (Stratagene, Cat. No. 740000) and Human Brain Reference RNA (Ambion, Cat. No. AM6050). As with any amplification, there is a risk of introducing bias into the experiment.
What kind of samples are recommended for hybridizing on NimbleGen gene expression arrays? We recommend using Cy3-labeled cDNA sample on our gene expression arrays. Please contact the Account Manager for your region to obtain a recommended protocol for preparing Cy3-labeled cDNA.
Data Analysis
What method of normalization does NimbleGen use for gene expression? We normalize expression data using quantile normalization. See Bolstad B, et al. (2003), A comparison of normalization methods for high density oligonucleotide array data based on bias and variance. Bioinformatics, 19:185-193.
How does NimbleGen generate gene calls from gene expression arrays? Gene calls are generated using the Robust Multichip Average (RMA) algorithm. See Irizarry R. et al. (2003), Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4:249-264.
What software do you recommend for further analysis of gene expression data? NimbleGen provides gene expression values in calls file that can be conveniently imported into multiple gene expression analysis software such as ArrayStar (DNAStar). Currently, with the purchase of a full service project, you will obtain a 30-day demo version of ArrayStar along with your data. Array delivery customers can also request this 30-day demo version from NimbleGen.
Can NimbleGen gene expression data be used with publicly available software packages? Several analysis packages that have been used with NimbleGen gene expression data are:
1. NMPP: Described in Bioinformatics (2006); 22: 2955 - 2957
2. MeV: Described in J. Exp. Biol. (2007); 210: 1507 - 1517.
3. R: Available at www.bioconductor.org, requires data in XYS format.
Array Service
How much RNA or cDNA do you need for gene expression analysis?
  Total RNA* mRNA* cDNA
Eukaryote 20μg 10μg 4μg
Class I Prokaryote 20μg - 4μg
Class II and III Prokaryotes 30μg - 8μg
*Please note that the NimbleGen procedure for sample processing does not include RNA amplification.
When I use NimbleGen full service option, what deliverables will I receive ? NimbleGen delivers the following data files and software to full service customer:
- Microarray Design file containing probe sequences (ndf)
- Gene Description file (ngd)
- Raw Data files (calls and pair)
- Normalized Data files (RMA_calls and RMA_pair)
- ArrayStar v2.0 expression analysis software.
Array Delivery
Can I purchase NimbleGen arrays for use in my own lab or core facility? Yes, all catalog and custom arrays are available for delivery.
Which gene expression designs are available for array delivery? All catalog designs currently listed on NimbleGen website and any custom design are available for delivery.
What kits are available for use with NimbleGen microarrays? NimbleGen offers Hybridization kits to facilitate efficient and specific hybridization to our expression arrays. See our Kits & Consumables web page for more details about these kits.
What resolution scanner do I need for scanning NimbleGen arrays? A scanner with 5 μm resolution is required.
Can I scan NimbleGen arrays using my Agilent scanner? Yes.
What software do I need to extract my data from a scanned image? NimbleGen offers array delivery customers with NimbleScan data extraction and analysis software.
Does NimbleGen offer training for array delivery customers? Yes, NimbleGen offers training at your lab or at our Madison, WI location. Please see our Training webpage for more details and a current schedule.
When I order microarrays from NimbleGen, what deliverables will I receive? For microarray orders, NimbleGen delivers the following items to the customer:
- NimbleGen arrays
- Complete User Guide
- Microarray Design file containing probe sequences (ndf)
- Gene Description file (ngd)
- ArrayStar expression analysis software (upon request)
How much sample do I need to run one microarray analysis in my lab? When using NimbleGen-recommended protocol for cDNA synthesis and labeling, 10 μg total RNA, 1 μg mRNA, or 1 μg cDNA is required for each array.
Eukaryotic Gene Expression Arrays
General Array Specs
Probe Length: 60mer
Feature Size: 16μm x 16μm
Array Size: 17.4mm x 13mm (1-plex format), 7.8mm x 5.7mm (4-plex format)
Slide Size: 1" x 3" (25 x 75mm)
Recommended Storage: Desiccated at room temperature.
Organism: Arabidopsis thaliana
Source: TAIR
Build: TAIR 6.0
Accession Numbers: NC_003070, NC_003071, NC_003074, NC_003075, NC_003075
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A4352001-00-01 Arabidopsis 60mer expr Arabidopsis thaliana 1-Plex Array
30,387
6
385,000
2
A4511001-00-01 ATH6 60mer expr X4 Arabidopsis thaliana 4-Plex Array
30,361
2
4 x 72,000
1
Organism: Caenorhabditis elegans
Source: Wormbase
Build: WS160
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A4354001-00-01 C elegans 60mer expr Caenorhabditis elegans 1-Plex Array
23,989
8
385,000
2
A4510001-00-01 CE WS160 60mer expr X4 Caenorhabditis elegans 4-Plex Array
23,336
3
4 x 72,000
1
Organism: Danio rerio
Source: Ensembl and UCSC
Build: Zv6
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A6183-00-01 2007-03-01 Zv6 60mer EXP Danio rerio 1-Plex Array
32,292
12
385,000
1
Organism: Drosophila melanogaster
Source: Flybase
Build: DM4.3
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A4351001-00-01 Drosophila 60mer expr Drosophila melanogaster 1-Plex Array
15,634
12
385,000
2
A4509001-00-01 DM4 3 60mer expr X4 Drosophila melanogaster 4-Plex Array
15,473
4
4 x 72,000
1
Organism: Homo sapiens
Source: NCBI
Build: HG18, Build 36
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A4542-00-01 HG18 60mer expr Homo sapiens 1-Plex Array
47,633
8
385,000
1
A4487001-00-01 HG18 60mer expr 4plex Homo sapiens 4-Plex Array
24,000
3
4 x 72,000
1
Organism: Mus musculus
Source: NCBI
Build: MM8
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A4543-00-01 MM8 60mer expr Mus musculus 1-Plex Array
42,586
9
385,000
1
A4486001-00-01 MM8 60mer expr X4 Mus musculus 4-Plex Array
25,631
3
4 x 72,000
1
Organism: Rattus norvegicus
Source: Ensembl
Build: RGSC 3.4
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A6184-00-01 2007-07-20 Rat X1 expr Rattus norvegicus 1-Plex Array
26,739
14
385,000
1
A6185-00-01 2007-07-20 Rat X4 expr Rattus norvegicus 4-Plex Array
26,208
3
4 x 72,000
1
Organism: Saccharomyces cerevisiae
Source: NCBI
Build: October 2003
Accession Numbers:NC_001133, NC_001134, NC_001135, NC_001136, NC_001137, NC_001138, NC_001139, NC_001140, NC_001141, NC_001142, NC_001143, NC_001144, NC_001145, NC_001146, NC_001147, NC_001148, NC_001224
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A4345001-00-01 TI4932 60mer Saccharomyces cerevisiae 1-Plex Array
5,868
20
385,000
3
A6186-00-01 TI4932 60mer expr X4 Saccharomyces cerevisiae 4-Plex Array
5,747
6
4 x 72,000
2
Organism: Schizosaccharomyces pombe
Source: NCBI
Build: June 2005
Accession Numbers: NC_003421, NC_003423, NC_003424
Catalog Number Design Name Description
Genes
Probes/ Target
Features
Replicates
A4346001-00-01 TI4896 60mer Schizosaccharomyces pombe 1-Plex Array
5,040
19
385,000
4
A6187-00-01 TI4896 60mer expr X4 Schizosaccharomyces pombe 4-Plex Array
4,997
7
4 x 72,000
2
Prokaryotic Gene Expression Arrays
Archaea
Kingdom: Archaea
Source: NCBI
Probe Length: 60mer
Feature Size: 16μm x 16μm
Array Size: 17.4mm x 13mm (1-plex format), 7.8mm x 5.7mm (4-plex format)
Slide Size: 1" x 3" (25 x 75mm)
Recommended Storage: Desiccated at room temperature.
4-Plex Option: Contact your Account Manager to order 4-plex gene expression arrays for any of these species.
Catalog Number Design Name Description Accession Numbers
Genes
Probes/ Target
Features
Replicates
A4431-00-01 TI272557 60mer Aeropyrum pernix K1 NC_000854
1,841
20
192,000
5
A4353-00-01 TI224325 60mer Archaeoglobus fulgidus DSM 4304 NC_000917
2,420
19
192,000
4
A4392-00-01 TI243232 60mer Methanocaldococcus jannaschii DSM 2661 NC_000909, NC_001732, NC_001733
1,786
20
192,000
5
A4423-00-01 TI267377 60mer Methanococcus maripaludis S2 NC_005791
1,722
20
192,000
5
A4305-00-01 TI190192 60mer Methanopyrus kandleri AV19 NC_003551
1,687
20
192,000
5
A4303-00-01 TI188937 60mer Methanosarcina acetivorans C2A NC_003552
4,540
16
385,000
5
A7240-00-01 080303 MacetC2A EXP X4 Methanosarcina acetivorans C2A NC_003552
4,433
8
4 x 72,000
2
A4429-00-01 TI269797 60mer Methanosarcina barkeri str. fusaro NC_007349, NC_007355
3,625
17
192,000
3
A4308-00-01 TI192952 60mer Methanosarcina mazei Go1 NC_003901
3,371
18
192,000
3
A4302-00-01 TI187420 60mer Methanothermobacter thermautotrophicus str. Delta H NC_000916
1,873
20
192,000
5
A4365-00-01 TI228908 60mer Nanoarchaeum equitans Kin4-M NC_005213
536
20
192,000
5
A4296-00-01 TI178306 60mer Pyrobaculum aerophilum str. IM2 NC_003364
2,605
18
192,000
4
A4300-00-01 TI186497 60mer Pyrococcus furiosus DSM 3638 NC_003413
2,125
17
192,000
5
A4511-00-01 TI69014 60mer Thermococcus kodakarensis KOD1 NC_006624
2,306
16
192,000
5
A4453-00-01 TI273075 60mer Thermoplasma acidophilum DSM 1728 NC_002578
1,482
20
192,000
5
A4454-00-01 TI273116 60mer Thermoplasma volcanium GSS1 NC_002689
1,499
20
192,000
5
A4414-00-01 TI263820 60mer Picrophilus torridus DSM 9790 NC_005877
1,535
20
192,000
5
A4446-00-01 TI272844 60mer Pyrococcus abyssi GE5 NC_000868, NC_001773
1,898
19
192,000
5
A4512-00-01 TI70601 60mer Pyrococcus horikoshii OT3 NC_000961
1,955
19
192,000
5
A4498-00-01 TI330779 60mer Sulfolobus acidocaldarius DSM 639 NC_007181
2,223
16
192,000
5
A4450-00-01 TI273057 60mer Sulfolobus solfataricus P2 NC_002754
2,977
12
192,000
5
A4451-00-01 TI273063 60mer Sulfolobus tokodaii str. 7 NC_003106
2,825
13
192,000
5
Bacteria
Kingdom: Bacteria
Source: NCBI
Probe Length: 60mer
Feature Size: 16μm x 16μm
Array Size: 17.4mm x 13mm (1-plex format), 7.8mm x 5.7mm (4-plex format)
Slide Size: 1" x 3" (25 x 75mm)
Recommended Storage: Desiccated at room temperature.
4-Plex Option: Contact your Account Manager to order 4-plex gene expression arrays for any of these species.
Catalog Number Design Name Description Accession Numbers
Genes
Probes/ Target
Features
Replicates
A4509-00-01 TI62977 60mer Acinetobacter sp. ADP1 NC_005966
3,325
14
192,000
4
A4292-00-01 TI176299 a 60mer Agrobacterium tumefaciens str. C58 NC_003304, NC_003305, NC_003306, NC_003308
5,402
14
385,000
5
A4293-00-01 TI176299 b 60mer Agrobacterium tumefaciens str. C58 NC_003062, NC_003063, NC_003064, NC_003065
5,288
18
385,000
4
A4385-00-01 TI240292 60mer Anabaena variabilis ATCC 29413 NC_007410, NC_007411, NC_007412, NC_007413
5,657
17
385,000
4
A4382-00-01 TI234826 60mer Anaplasma marginale str. St. Maries NC_004842
949
20
192,000
5
A4352-00-01 TI224324 60mer Aquifex aeolicus VF5 NC_000918, NC_001880
1,560
20
192,000
5
A4317-00-01 TI198094 60mer Bacillus anthracis str. Ames NC_003997
5,311
18
385,000
4
A4408-00-01 TI261594 60mer Bacillus anthracis str. 'Ames Ancestor' NC_007322, NC_007323, NC_007530
5,617
17
385,000
4
A6704-00-01 071112 Bant AmesAncester EXP X4 Bacillus anthracis str. 'Ames Ancestor' NC_007322, NC_007323, NC_007530
5,615
6
4 x 72,000
2
A4407-00-01 TI260799 60mer Bacillus anthracis str. Sterne NC_005945
5,287
18
385,000
4
A4346-00-01 TI222523 60mer Bacillus cereus ATCC 10987 NC_003909, NC_005707
5,844
13
385,000
5
A4361-00-01 TI226900 60mer Bacillus cereus ATCC 14579 NC_004721, NC_004722
5,255
18
385,000
4
A4469-00-01 TI288681 60mer Bacillus cereus E33L NC_006274, NC_007103, NC_007104, NC_007105, NC_007106, NC_007107
5,641
17
385,000
4
A4510-00-01 TI66692 60mer Bacillus clausii KSM-K16 NC_006582
4,096
18
385,000
5
A4432-00-01 TI272558 60mer Bacillus halodurans C-125 NC_002570
4,066
18
385,000
5
A4458-00-01 TI279010 a 60mer Bacillus licheniformis ATCC 14580 NC_006270
4,152
18
385,000
5
A4459-00-01 TI279010 b 60mer Bacillus licheniformis ATCC 14580 NC_006322
4,196
18
385,000
5
A4349-00-01 TI224308 60mer Bacillus subtilis subsp. subtilis str. 168 NC_000964
4,105
18
385,000
5
A6696-00-01 071112 Bsub 168 EXP X4 Bacillus subtilis subsp. subtilis str. 168 NC_000964
4,104
8
4 x 72,000
2
A4461-00-01 TI281309 60mer Bacillus thuringiensis serovar konkukian str. 97-27 NC_005957, NC_006578
5,197
18
385,000
4
A4433-00-01 TI272559 60mer Bacteroides fragilis NCTC 9343 NC_003228, NC_006873
4,231
18
385,000
5
A6705-00-01 071112 Bfra NCTC9343 EXP X4 Bacteroides fragilis NCTC 9343 NC_003228, NC_006873
4,229
8
4 x 72,000
2
A4476-00-01 TI295405 60mer Bacteroides fragilis YCH46 NC_006297, NC_006347
4,625
16
385,000
5
A4360-00-01 TI226186 60mer Bacteroides thetaiotaomicron VPI-5482 NC_004663, NC_004703
4,816
19
385,000
4
A4466-00-01 TI283166 60mer Bartonella henselae str. Houston-1 NC_005956
1,488
20
192,000
5
A4465-00-01 TI283165 60mer Bartonella quintana str. Toulouse NC_005955
1,142
20
192,000
5
A4418-00-01 TI264462 60mer Bdellovibrio bacteriovorus HD100 NC_005363
3,587
13
192,000
4
A4329-00-01 TI206672 60mer Bifidobacterium longum NCC2705 NC_004307, NC_004943
1,729
20
192,000
5
A4356-00-01 TI224326 60mer Borrelia burgdorferi B31 NC_000948, NC_000949, NC_000950, NC_000951, NC_000952, NC_000953, NC_000954, NC_000955, NC_000956, NC_000957, NC_001318, NC_001849, NC_001850, NC_001851, NC_001852, NC_001853, NC_001854, NC_001855, NC_001856, NC_001857, NC_001903, NC_001904
1,639
20
192,000
5
A4470-00-01 TI290434 60mer Borrelia garinii PBi NC_006128, NC_006129, NC_006156, NT_108227, NT_108228, NT_108229, NT_108230, NT_108231, NT_108232, NT_108233, NT_108234, NT_108235, NT_108236, NT_108237, NT_108238, NT_108239, NT_108240, NT_108241, NT_108242, NT_108243, NT_108244, NT_108245, NT_108246, NT_108247, NT_108248, NT_108249, NT_108250, NT_108251, NT_108252, NT_108253, NT_108254, NT_108255, NT_108256, NT_108257, NT_108258, NT_108259, NT_108260, NT_108261, NT_108262, NT_108263
1,270
20
192,000
6
A4409-00-01 TI262698 60mer Brucella abortus biovar 1 str. 9-941 NC_006932, NC_006933
3,085
12
192,000
5
A4357-00-01 TI224914 60mer Brucella melitensis 16M NC_003317, NC_003318
3,198
19
192,000
3
A4505-00-01 TI359391 60mer Brucella melitensis biovar Abortus 2308 NC_007618, NC_007624
3,034
12
192,000
5
A4325-00-01 TI204722 60mer Brucella suis 1330 NC_004310, NC_004311
3,271
19
192,000
3
A4268-00-01 TI107806 60mer Buchnera aphidicola str. APS (Acyrthosiphon pisum) NC_002252, NC_002253, NC_002528
574
20
192,000
5
A4358-00-01 TI224915 60mer Buchnera aphidicola str. Bp (Baizongia pistaciae) NC_004545
504
20
192,000
5
A4321-00-01 TI198804 60mer Buchnera aphidicola str. Sg (Schizaphis graminum) NC_004061
546
20
192,000
5
A4311-00-01 TI195099 60mer Campylobacter jejuni RM1221 NC_003912
1,838
20
192,000
5
A4307-00-01 TI192222 60mer Campylobacter jejuni subsp. jejuni NCTC 11168 NC_002163
1,629
20
192,000
5
A7409-00-01 080328 C jejuni NCTC 11168 expr Campylobacter jejuni subsp. jejuni NCTC 11168 NC_002163
1,634
22
4 x 72,000
2
A4324-00-01 TI203907 60mer Candidatus Blochmannia floridanus NC_005061
583
20
192,000
5
A4471-00-01 TI291272 60mer Candidatus Blochmannia pennsylvanicus str. BPEN NC_007292
610
20
192,000
5
A4499-00-01 TI335992 60mer Candidatus Pelagibacter ubique HTCC1062 NC_007205
1,354
20
192,000
5
A4416-00-01 TI264201 60mer Candidatus Protochlamydia amoebophila UWE25 NC_005861
2,031
18
192,000
5
A4399-00-01 TI246194 60mer Carboxydothermus hydrogenoformans Z-2901 NC_007503
2,620
14
192,000
5
A4389-00-01 TI243161 60mer Chlamydia muridarum Nigg NC_002182, NC_002620
911
20
192,000
5
A4487-00-01 TI315277 60mer Chlamydia trachomatis A/HAR-13 NC_007429, NC_007430
919
20
192,000
5
A4434-00-01 TI272561 60mer Chlamydia trachomatis D/UW-3/CX NC_000117
895
20
192,000
5
A4341-00-01 TI218497 60mer Chlamydophila abortus S26/3 NC_004552
932
20
192,000
5
A4363-00-01 TI227941 60mer Chlamydophila caviae GPIC NC_003361, NC_004720
1,005
20
192,000
5
A4273-00-01 TI115711 60mer Chlamydophila pneumoniae AR39 NC_002179
1,112
20
192,000
5
A4274-00-01 TI115713 60mer Chlamydophila pneumoniae CWL029 NC_000922
1,052
20
192,000
5
A4277-00-01 TI138677 60mer Chlamydophila pneumoniae J138 NC_002491
1,069
20
192,000
5
A4297-00-01 TI182082 60mer Chlamydophila pneumoniae TW-183 NC_005043
1,113
20
192,000
5
A4502-00-01 TI340177 60mer Chlorobium chlorochromatii CaD3 NC_007514
2,002
18
192,000
5
A4310-00-01 TI194439 60mer Chlorobium tepidum TLS NC_002932
2,252
16
192,000
5
A4435-00-01 TI272562 60mer Clostridium acetobutylicum ATCC 824 NC_001988, NC_003030
3,848
20
385,000
5
A7307-00-01 080303 TI272562 60mer expr X4 Clostridium acetobutylicum ATCC 824 NC_001988, NC_003030
3,847
9
4 x 72,000
2
A4312-00-01 TI195102 60mer Clostridium perfringens str. 13 NC_003042, NC_003366
2,723
17
192,000
4
A4336-00-01 TI212717 60mer Clostridium tetani E88 NC_004557, NC_004565
2,432
19
192,000
4
A4286-00-01 TI167879 60mer Colwellia psychrerythraea 34H NC_003910
4,910
19
385,000
4
A4403-00-01 TI257309 60mer Corynebacterium diphtheriae NCTC 13129 NC_002935
2,272
16
192,000
5
A4315-00-01 TI196627 60mer Corynebacterium glutamicum ATCC 13032 NC_003450, NC_006958
6,050
12
385,000
5
A4362-00-01 TI227377 60mer Coxiella burnetii RSA 493 NC_002971, NC_004704
2,052
18
192,000
5
A4281-00-01 TI159087 60mer Dechloromonas aromatica RCB NC_007298
4,171
18
385,000
5
A4390-00-01 TI243164 60mer Dehalococcoides ethenogenes 195 NC_002936
1,580
20
192,000
5
A4402-00-01 TI255470 60mer Dehalococcoides sp. CBDB1 NC_007356
1,458
20
192,000
5
A4295-00-01 TI177439 60mer Desulfotalea psychrophila LSv54 NC_006138, NC_006139, NC_006140
3,234
19
192,000
3
A4330-00-01 TI207559 60mer Desulfovibrio desulfuricans G20 NC_007519
3,775
20
385,000
5
A4428-00-01 TI269484 60mer Ehrlichia canis str. Jake NC_007354
925
20
192,000
5
A4484-00-01 TI302409 60mer Ehrlichia ruminantium str. Gardel NC_006831
950
20
192,000
5
A4400-00-01 TI254945 a 60mer Ehrlichia ruminantium str. Welgevonden NC_005295
888
20
192,000
5
A4401-00-01 TI254945 b 60mer Ehrlichia ruminantium str. Welgevonden NC_006832
958
20
192,000
5
A4359-00-01 TI226185 60mer Enterococcus faecalis V583 NC_004668, NC_004669, NC_004670, NC_004671
3,265
19
192,000
3
A4339-00-01 TI218491 60mer Erwinia carotovora subsp. atroseptica SCRI1043 NC_004547
4,472
17
385,000
5
A4322-00-01 TI199310 60mer Escherichia coli CFT073 NC_004431
5,379
14
385,000
5
A4516-00-01 TI83333 60mer Escherichia coli K12 NC_000913
4,237
18
385,000
5
A6697-00-01 071112 Ecoli K12 EXP X4 Escherichia coli K12 NC_000913
4,254
8
4 x 72,000
2
A4517-00-01 TI83334 60mer Escherichia coli O157:H7 NC_002127, NC_002128, NC_002695, NC_007414
5,440
14
385,000
5
A4278-00-01 TI155864 60mer Escherichia coli O157:H7 EDL933 NC_002655
5,324
18
385,000
4
A6698-00-01 071112 Ecoli H7EDL933 EXP X4 Escherichia coli O157:H7 EDL933 NC_007414, NC_002655
5,223
6
4 x 72,000
2
A4294-00-01 TI177416 60mer Francisella tularensis subsp. tularensis SCHU S4 NC_006570
1,603
20
192,000
5
A4306-00-01 TI190304 60mer Fusobacterium nucleatum subsp. nucleatum ATCC 25586 NC_003454
2,067
18
192,000
5
A4384-00-01 TI235909 60mer Geobacillus kaustophilus HTA426 NC_006509, NC_006510
3,540
13
192,000
4
A4430-00-01 TI269799 60mer Geobacter metallireducens GS-15 NC_007515, NC_007517
3,532
13
192,000
4
A4391-00-01 TI243231 60mer Geobacter sulfurreducens PCA NC_002939
3,446
18
192,000
3
A4381-00-01 TI233412 60mer Haemophilus ducreyi 35000HP NC_002940
1,717
20
192,000
5
A4462-00-01 TI281310 60mer Haemophilus influenzae 86-028NP NC_007146
1,791
20
192,000
5
A4513-00-01 TI71421 60mer Haemophilus influenzae Rd KW20 NC_000907
1,657
20
192,000
5
A4504-00-01 TI349521 60mer Hahella chejuensis KCTC 2396 NC_007645
6,778
14
385,000
4
A4383-00-01 TI235279 60mer Helicobacter hepaticus ATCC 51449 NC_004917
1,875
20
192,000
5
A4519-00-01 TI85962 60mer Helicobacter pylori 26695 NC_000915
1,576
20
192,000
5
A4520-00-01 TI85963 60mer Helicobacter pylori J99 NC_000921
1,491
20
192,000
5
A4467-00-01 TI283942 60mer Idiomarina loihiensis L2TR NC_006512
2,628
14
192,000
5
A4436-00-01 TI272621 60mer Lactobacillus acidophilus NCFM NC_006814
1,864
20
192,000
5
A4404-00-01 TI257314 60mer Lactobacillus johnsonii NCC 533 NC_005362
1,821
20
192,000
5
A4343-00-01 TI220668 60mer Lactobacillus plantarum WCFS1 NC_004567, NC_006375, NC_006376, NC_006377
3,059
12
192,000
5
A4486-00-01 TI314315 60mer Lactobacillus sakei subsp. sakei 23K NC_007576
1,880
19
192,000
5
A4437-00-01 TI272623 60mer Lactococcus lactis subsp. lactis Il1403 NC_002662
2,321
16
192,000
5
A4477-00-01 TI297245 60mer Legionella pneumophila str. Lens NC_006366, NC_006369
2,934
16
192,000
4
A4478-00-01 TI297246 60mer Legionella pneumophila str. Paris NC_006365, NC_006368
3,166
19
192,000
3
A4438-00-01 TI272624 60mer Legionella pneumophila subsp. pneumophila str. Philadelphia 1 NC_002942
2,942
12
192,000
5
A4424-00-01 TI267671 60mer Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 NC_005823, NC_005824
3,658
17
192,000
3
A4304-00-01 TI189518 60mer Leptospira interrogans serovar Lai str. 56601 NC_004342, NC_004343
4,727
16
385,000
5
A4439-00-01 TI272626 60mer Listeria innocua Clip11262 NC_003212, NC_003383
3,043
12
192,000
5
A4287-00-01 TI169963 60mer Listeria monocytogenes EGD-e NC_003210
2,846
13
192,000
5
A4422-00-01 TI265669 60mer Listeria monocytogenes str. 4b F2365 NC_002973
2,821
13
192,000
5
A7410-00-01 080328 L monocytogenes 4bF2365 expr Listeria monocytogenes str. 4b F2365 NC_002973
2,821
12
4 x 72,000
2
A4345-00-01 TI221988 60mer Mannheimia succiniciproducens MBEL55E NC_006300
2,380
19
192,000
4
A4421-00-01 TI265311 60mer Mesoplasma florum L1 NC_006055
682
20
192,000
5
A4420-00-01 TI264732 60mer Moorella thermoacetica ATCC 39073 NC_007644
2,465
19
192,000
4
A4440-00-01 TI272631 60mer Mycobacterium leprae TN NC_002677
1,605
20
192,000
5
A4501-00-01 TI340047 60mer Mycoplasma capricolum subsp. capricolum ATCC 27343 NC_007633
812
20
192,000
5
A4380-00-01 TI233150 60mer Mycoplasma gallisepticum R NC_004829
726
20
192,000
5
A4394-00-01 TI243273 60mer Mycoplasma genitalium G37 NC_000908
484
20
192,000
5
A4475-00-01 TI295358 60mer Mycoplasma hyopneumoniae 232 NC_006360
691
20
192,000
5
A4411-00-01 TI262722 60mer Mycoplasma hyopneumoniae 7448 NC_007332
663
20
192,000
5
A4410-00-01 TI262719 60mer Mycoplasma hyopneumoniae J NC_007295
665
20
192,000
5
A4426-00-01 TI267748 60mer Mycoplasma mobile 163K NC_006908
633
20
192,000
5
A4441-00-01 TI272632 60mer Mycoplasma mycoides subsp. mycoides SC str. PG1 NC_005364
1,016
20
192,000
5
A4442-00-01 TI272633 60mer Mycoplasma penetrans HF-2 NC_004432
1,037
20
192,000
5
A4443-00-01 TI272634 60mer Mycoplasma pneumoniae M129 NC_000912
689
20
192,000
5
A4444-00-01 TI272635 60mer Mycoplasma pulmonis UAB CTIP NC_002771
782
20
192,000
5
A4412-00-01 TI262723 60mer Mycoplasma synoviae 53 NC_007294
672
20
192,000
5
A4386-00-01 TI242231 60mer Neisseria gonorrhoeae FA 1090 NC_002946
2,002
18
192,000
5
A4275-00-01 TI122586 60mer Neisseria meningitidis MC58 NC_003112
2,063
18
192,000
5
A4276-00-01 TI122587 60mer Neisseria meningitidis Z2491 NC_003116
2,065
18
192,000
5
A4494-00-01 TI323261 60mer Nitrosococcus oceani ATCC 19707 NC_007483, NC_007484
3,017
12
192,000
5
A4364-00-01 TI228410 60mer Nitrosomonas europaea ATCC 19718 NC_004757
2,461
19
192,000
4
A7306-00-01 080303 TI228410 60mer expr X4 Nitrosomonas europaea ATCC 19718 NC_004757
2,368
14
4 x 72,000
2
A4495-00-01 TI323848 60mer Nitrosospira multiformis ATCC 25196 NC_007614, NC_007615, NC_007616, NC_007617
2,805
13
192,000
5
A4267-00-01 TI103690 60mer Nostoc sp. PCC 7120 NC_003240, NC_003241, NC_003267, NC_003270, NC_003272, NC_003273, NC_003276
6,130
12
385,000
5
A4344-00-01 TI221109 60mer Oceanobacillus iheyensis HTE831 NC_004193
3,500
13
192,000
4
A4413-00-01 TI262768 60mer Onion yellows phytoplasma OY-M NC_005303
754
20
192,000
5
A4445-00-01 TI272843 60mer Pasteurella multocida subsp. multocida str. Pm70 NC_002663
2,015
18
192,000
5
A4500-00-01 TI338963 60mer Pelobacter carbinolicus DSM 2380 NC_007498
3,118
12
192,000
5
A4491-00-01 TI319225 60mer Pelodictyon luteolum DSM 273 NC_007512
2,083
18
192,000
5
A4479-00-01 TI298386 60mer Photobacterium profundum SS9 NC_005871, NC_006370, NC_006371
5,491
14
385,000
5
A4393-00-01 TI243265 60mer Photorhabdus luminescens subsp. laumondii TTO1 NC_005126
4,683
16
385,000
5
A4387-00-01 TI242619 60mer Porphyromonas gingivalis W83 NC_002950
1,909
19
192,000
5
A4514-00-01 TI74546 60mer Prochlorococcus marinus str. MIT 9312 NC_007577
1,809
20
192,000
5
A4515-00-01 TI74547 60mer Prochlorococcus marinus str. MIT 9313 NC_005071
2,265
16
192,000
5
A4508-00-01 TI59920 60mer Prochlorococcus marinus str. NATL2A NC_007335
1,890
19
192,000
5
A4285-00-01 TI167539 60mer Prochlorococcus marinus subsp. marinus str. CCMP1375 NC_005042
1,882
19
192,000
5
A4507-00-01 TI59919 60mer Prochlorococcus marinus subsp. pastoris str. CCMP1986 NC_005072
1,712
20
192,000
5
A4425-00-01 TI267747 60mer Propionibacterium acnes KPA171202 NC_006085
2,297
16
192,000
5
A4497-00-01 TI326442 60mer Pseudoalteromonas haloplanktis TAC125 NC_007481, NC_007482
3,486
13
192,000
4
A4328-00-01 TI205922 60mer Pseudomonas fluorescens PfO-1 NC_007492
5,736
13
385,000
5
A7241-00-01 080306 Pfluorescens Pf0 1 EXP Pseudomonas fluorescens PfO-1 NC_007492
5,733
6
4 x 72,000
2
A4419-00-01 TI264730 60mer Pseudomonas syringae pv. phaseolicola 1448A NC_005773, NC_007274, NC_007275
5,170
18
385,000
4
A4326-00-01 TI205918 60mer Pseudomonas syringae