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CGH - Human Designs


Comparative Genomic Hybridization (CGH) measures DNA copy number differences between a test and reference genome. Roche NimbleGen offers ultra-high resolution CGH/CNV arrays with up to 2.1 million probes for comprehensive analysis of DNA copy number variation. In addition to the 2.1M array format, multiplex arrays are available for higher throughput and cost-effective analysis of either 3 or 12 samples on a single slide. Roche NimbleGen offers several types of CGH/CNV arrays for a variety of organisms to meet your specific research needs:

  • Cytogenetics (CGX) Arrays for genome-wide analysis of DNA copy number changes with a subset of probes focused in disease-associated regions
  • CNV Arrays for high-resolution CNV discovery and association studies
  • CGH Whole-Genome Tiling Arrays for comprehensive, unbiased analysis of DNA copy number changes.
  • CGH Whole-Genome Exon-Focused Arrays for genome-wide analysis of DNA copy number changes with a subset of probes focused in exon regions.
  • CGH Custom Targeted Arrays for DNA copy number analysis of a whole genome, a single chromosomal region, or multiple loci of interest.
Cytogenetics
CGX-3 Array designed by Signature Genomics
New 4 Slide Packs!
Format: 3 arrays per slide
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Pack Size Version Probe Length Median Probe Spacing
CGX-3 Whole-Genome Array Delivery Workflow. Click to learn more... 05986834001 2 Slides 1.0 60mer Variable
Delivery Workflow. Click to learn more... 05947987001 4 Slides
CGX-12 Array designed by Signature Genomics
New 2 and 4 Slide Packs!
Format: 12 arrays per slide
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Pack Size Version Probe Length Median Probe Spacing
CGX-12 Whole-Genome Array Delivery Workflow. Click to learn more... 05986869001 1 Slide 1.0 60mer Variable
Delivery Workflow. Click to learn more... 05947995001 2 Slides
Delivery Workflow. Click to learn more... 05948002001 4 Slides
CNV
2.1M CNV
New 5 Slide Packs!
NimbleGen 2.1M Array Format: 2.1M
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Pack Size Version Probe Length Backbone Median Probe Spacing
CNV Array Delivery Workflow. Click to learn more... 05913152001 1 Slide 1.0 50-75mer 1239bp
Delivery Workflow. Click to learn more... 05913179001 5 Slides
3x720K CNV
New 5 Slide Packs!
NimbleGen 3x720K Array Format: 3x720K
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Pack Size Version Probe Length Backbone Median Probe Spacing
CNV Array Delivery Workflow. Click to learn more... 05913209001 1 Slide 1.0 50-75mer 4750bp
Delivery Workflow. Click to learn more... 05913217001 5 Slides
Whole-Genome Tiling
2.1M Whole-Genome Tiling
NimbleGen 2.1M Array Format: 2.1M
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Design Name Version Probe Length Median Probe Spacing
Whole Genome Tiling Array Delivery Workflow. Click to learn more... 05541921001 Human CGH 2.1M WG-T v2.0D Arr Del 2.0D 60mer 1169bp
Service Workflow. Click to learn more... 05543991001 Human CGH 2.1M WG-T v2.0D Arr Ser
3x720K Whole-Genome Tiling
NimbleGen 3x720K Array Format: 3x720K
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Design Name Version Probe Length Median Probe Spacing
Whole Genome Tiling Array Delivery Workflow. Click to learn more... 05520797001 Human CGH 3x720K WG-T v3.0 Arr Del 3.0 60mer 2509bp
Service Workflow. Click to learn more... 05520860001 Human CGH 3x720K WG-T v3.0 Arr Ser
Whole Genome Tiling Array Delivery Workflow. Click to learn more... 05542065001 Human CGH 3x720K WG-T v2.0 Arr Del 2.0 60mer 3877bp
Service Workflow. Click to learn more... 05544114001 Human CGH 3x720K WG-T v2.0 Arr Ser
12x135K Whole-Genome Tiling
NimbleGen 12x135K Array Format: 12x135K
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Design Name Version Probe Length Median Probe Spacing
Whole Genome Tiling Array Delivery Workflow. Click to learn more... 05520878001 Human CGH 12x135K WG-T v3.0 Arr Del 3.0 60mer 12524bp
Service Workflow. Click to learn more... 05520886001 Human CGH 12x135K WG-T v3.0 Arr Ser
Whole Genome Tiling Array Delivery Workflow. Click to learn more... 05542219001 Human CGH 12x135K WG-T v2.0 Arr Del 2.0 60mer 21500bp
Service Workflow. Click to learn more... 05544254001 Human CGH 12x135K WG-T v2.0 Arr Ser
385K Whole-Genome Tiling
NimbleGen 385K Array Format: 385K
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Design Name Version Probe Length Median Probe Spacing
Whole Genome Tiling Array Delivery Workflow. Click to learn more... 05542243001 Human CGH 385K WG-T v2.0 Arr Del 2.0 60mer 7073bp
Service Workflow. Click to learn more... 05544297001 Human CGH 385K WG-T v2.0 Arr Ser
Whole Genome Tiling Array Delivery or Service Workflow. Click to learn more... B4366-00-01 HG18 CGH 385K WG Tiling v1.0 1.0 50-75mer 6270bp
4x72K Whole-Genome
NimbleGen 4x72K Array Format: 4x72K
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Design Name Version Probe Length Median Probe Spacing
Whole Genome Tiling Array Delivery or Service Workflow. Click to learn more... B6925-00-01 HG18 CGH 4x72K WG Tiling v2.0 2.0 60mer 40046bp
Whole-Genome Exon-Focused
NimbleGen 3x720K Array Format: 3x720K
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Design Name Version Probe Length Median Probe Spacing
Whole Genome Exon-Focused Array Delivery Workflow. Click to learn more... 05542073001 Human CGH 3x720K Exon Foc Arr Del 1.0 50-75mer Variable
Service Workflow. Click to learn more... 05544122001 Human CGH 3x720K Exon Foc Arr Ser
Chromosome-Specific
NimbleGen 385K Array Format: 385K
Source: UCSC
Build: HG18, NCBI 36
Recommended Storage: Store arrays desiccated at room temperature.
Description Delivery Workflow. Click to learn more... Catalog Number Design Name Probe Length Median Probe Spacing
Chromosome 1 Tiling Array Delivery or Service Workflow. Click to learn more... B3732001-00-01 HG18 CHR1 FT 50-75mer 531
Chromosome 2 Tiling Array Delivery or Service Workflow. Click to learn more... B3733001-00-01 HG18 CHR2 FT 50-75mer 575
Chromosome 3 Tiling Array Delivery or Service Workflow. Click to learn more... B3734001-00-01 HG18 CHR3 FT 50-75mer 475
Chromosome 4 Tiling Array Delivery or Service Workflow. Click to learn more... B3735001-00-01 HG18 CHR4 FT 50-75mer 450
Chromosome 5 Tiling Array Delivery or Service Workflow. Click to learn more... B3736001-00-01 HG18 CHR5 FT 50-75mer 425
Chromosome 6 Tiling Array Delivery or Service Workflow. Click to learn more... B3737001-00-01 HG18 CHR6 FT 50-75mer 404
Chromosome 7 Tiling Array Delivery or Service Workflow. Click to learn more... B3738001-00-01 HG18 CHR7 FT 50-75mer 365
Chromosome 8 Tiling Array Delivery or Service Workflow. Click to learn more... B3739001-00-01 HG18 CHR8 FT 50-75mer 341
Chromosome 9 Tiling Array Delivery or Service Workflow. Click to learn more... B3740001-00-01 HG18 CHR9 FT 50-75mer 255
Chromosome 10 Tiling Array Delivery or Service Workflow. Click to learn more... B3741001-00-01 HG18 CHR10 FT 50-75mer 305
Chromosome 11 Tiling Array Delivery or Service Workflow. Click to learn more... B3742001-00-01 HG18 CHR11 FT 50-75mer 310
Chromosome 12 Tiling Array Delivery or Service Workflow. Click to learn more... B3743001-00-01 HG18 CHR12 FT 50-75mer 310
Chromosome 13 Tiling Array Delivery or Service Workflow. Click to learn more... B3744001-00-01 HG18 CHR13 FT 50-75mer 225
Chromosome 14 Tiling Array Delivery or Service Workflow. Click to learn more... B3745001-00-01 HG18 CHR14 FT 50-75mer 200
Chromosome 15 Tiling Array Delivery or Service Workflow. Click to learn more... B3746001-00-01 HG18 CHR15 FT 50-75mer 175
Chromosome 16 Tiling Array Delivery or Service Workflow. Click to learn more... B3747001-00-01 HG18 CHR16 FT 50-75mer 165
Chromosome 17 Tiling Array Delivery or Service Workflow. Click to learn more... B3748001-00-01 HG18 CHR17 FT 50-75mer 160
Chromosome 18 Tiling Array Delivery or Service Workflow. Click to learn more... B3749001-00-01 HG18 CHR18 FT 50-75mer 170
Chromosome 19 Tiling Array Delivery or Service Workflow. Click to learn more... B3750001-00-01 HG18 CHR19 FT 50-75mer 105
Chromosome 20 Tiling Array Delivery or Service Workflow. Click to learn more... B3751001-00-01 HG18 CHR20 FT 50-75mer 134
Chromosome 21 Tiling Array Delivery or Service Workflow. Click to learn more... B3752001-00-01 HG18 CHR21 FT 50-75mer 70
Chromosome 22 Tiling Array Delivery or Service Workflow. Click to learn more... B3753001-00-01 HG18 CHR22 FT 50-75mer 65
Chromosome X Tiling Array Delivery or Service Workflow. Click to learn more... B3754001-00-01 HG18 CHRX FT 50-75mer 340
Chromosome Y Tiling Array Delivery or Service Workflow. Click to learn more... B3755001-00-01 HG18 CHRY FT 50-75mer 20
Custom

Please contact your Sales representative to Request a Quote or contact Technical Support with any questions about CGH custom array designs.

  NEW!
NimbleGen 2.1M Array
2.1M		 NEW!
NimbleGen 3x720K Array
3x720K		 NEW!
NimbleGen 12x135K Array
12x135K		 NimbleGen 385K Array
385K		 NimbleGen 4x72K Array
4x72K
Number of Arrays per Slide 1 3 12 1 4
Total Number of Probes 2,100,000 3 x
720,000		 12 x
135,000		 385,000 4 x
72,000
Source Customer-defined
Build Customer-defined
Probe Length 50-75mer
Probe Spacing Uniform or mixed-density
Design Options Whole-genome, single or multiple chromosomal regions per array.

To learn more about NimbleGen Array formats, please visit our Array Products page.

Literature

For a complete listing of literature covering all Roche NimbleGen products and services please visit our literature page.

FAQ
General
How is NimbleGen array technology different from other platforms? NimbleGen manufactures high-density DNA microarrays using its proprietary Maskless Array Synthesizer (MAS) technology. At the heart of the system is a Digital Micromirror Device (DMD) that uses a solid-state array of miniature aluminum mirrors to create “virtual masks” that replace the physical chromium masks used in traditional arrays. The DMD directs a pattern of UV light projected onto a microscope slide, which when coupled with UV-mediated DNA synthesis in a parallel, combinatorial manner, can generate up to 2.1 million unique probes on a single microarray.
How many features are included on a single array? Individual arrays are available in variety of formats with up to 2.1 million features.
Does NimbleGen offer CGH arrays in multiplex formats? Yes, we offer Human Whole Genome and custom eukaryotic arrays in 3x720K, 12x135K, and 4x72K formats.
What is the resolution of your arrays? Effective resolution varies according to the spacing of individual probes (generally 5-10x the median probe spacing). Our human whole-genome arrays enable resolution down to ~5kb on the 2.1M format. Custom targeted arrays enable higher density probe spacing in specified regions and detection of DNA copy number changes down to exon-level resolution.
Do NimbleGen array designs include non-coding regions of the genome? Yes - NimbleGen offers unbiased whole-genome tiling designs that include genic and intergenic regions.
Can NimbleGen design an array tailored to my specific research needs? Yes - specify genomic regions of interest and NimbleGen will design a custom array to meet your research needs.
What resolution scanner do I need? The NimbleGen MS 200 scanner set at 5 micron resolution is optimal for 385K arrays. The NimbleGen MS 200 scanner set at 2 micron resolution is optimal for 2.1M arrays.
Can NimbleGen CGH distinguish single vs. multi-copy amplifications? Yes.
Sample Processing
What are the sample requirements for CGH? 1-5μg of high-quality unamplified gDNA is required.
What protocol does NimbleGen recommend for DNA isolation? Any protocol that provides high-quality gDNA.
What reference sample should I use? There are several options for choosing a reference sample. For example, for cancer studies choose an individual's own DNA (e.g. tumor vs. germline), DNA from a single individual, or pooled DNA from several individuals (NimbleGen can provide pooled male or female DNA).
Array Design
Are NimbleGen designs up-to-date with the latest genome builds? Yes, NimbleGen technology allows the rapid creation of new array designs based on the latest genome sequence.
Are designs based on earlier genome builds available? Yes.
Does NimbleGen offer non-human CGH designs? Yes - NimbleGen offers whole-genome designs for a wide range of organisms. In addition, NimbleGen can create custom designs for any genome (or region of genome) for which high-quality sequence is available.
Does NimbleGen use only non-unique probes? Generally only unique probes are included in array designs. However, NimbleGen Human Whole Genome v2.0 array designs contain non-unique probes for enhanced coverage of low-copy number repeat regions of the genome (e.g. segmental duplication).
Data Analysis
What software do I need to process NimbleGen CGH arrays in my own lab? Roche NimbleGen's NimbleScan and SignalMap software are required.
What software do I need to view my full service CGH data? Roche NimbleGen's SignalMap software is required for viewing CGH data in GFF format. A free, 30-day demo version of SignalMap is available for download.
Can I analyze my data using other software programs? Yes - NimbleGen data can be viewed and analyzed using a variety of commercially available software programs.
Can I view my data in the UCSC genome browser? Yes.
How do I get gene annotation information? NimbleGen provides a gene annotation track corresponding to your design. Additional annotation tracks can be viewed in the UCSC genome browser.
Array Delivery
Can I purchase NimbleGen arrays for use in my own lab or core facility? Yes.
Which CGH designs are available for array delivery? Whole-Genome and custom targeted array designs in any available array format.
What kits are availble for use with NimbleGen CGH microarrays? NimbleGen Hybridization, Wash Buffer, Array Processing Accessories, and Sample Tracking Control kits. Learn more about our kits...
Can I scan NimbleGen arrays using my Agilent scanner? The 5 micron Agilent scanner can work with NimbleGen arrays.
Does Roche NimbleGen offer training for array delivery customers? Yes, Roche NimbleGen Workshops are intensive, 2 to 4-day training programs designed to provide instruction in both the theory and practice of genomic and epigenomic research techniques using Roche NimbleGen high-density DNA microarrays. Click here to find out more.
Can I re-use NimbleGen CGH arrays? Roche NimbleGen offers a NimbleGen Array Reuse Kit that enables the stripping of NimbleGen CGH arrays so that they can be re-hybridized with newly-labeled test and reference genomic DNA. The success of array re-use is highly variable, depending on sample composition and hybridization conditions. Therefore, Roche NimbleGen does not guarantee the performance of the NimbleGen Array Reuse Kit.
 

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