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| Recent Webinars |
Casey Matthews
Research Associate II
NCI-CGF Scientific Operations Team |
May 6, 2010 |
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Targeted Re-Sequencing Made Easy: A Core Laboratory’s Experience with NimbleGen SeqCap EZ
From low throughput preparation of sample libraries to keeping the sequencing pipeline full, target enrichment and library preparation for next-generation sequencing has presented significant challenges at the Core Genotyping Facility at National Cancer Institute (NCI-CGF). Through key process optimization to library preparation coupled with the incorporation of NimbleGen SeqCap EZ, a solution-based enrichment technology, NCI-CGF has overcome the throughput challenges and increased capacity to manually enrich and process multiple 96-well plates. These improvements have proven to be significant, decreasing project turn-around times from one month to one week, opening the possibility of an automated solution, and decreasing costs while increasing efficiency for next generation targeted re-sequencing projects.
Also presented in this webinar are target re-sequencing results from a genomic region associated with prostate cancer risk in 78 research samples. The results of this study have generated a detailed map of common genetic variations in chr19q13.33, and these variations should facilitate fine-mapping the association signal as well as determining the contribution of this locus to prostate cancer risk and regulation of prostate specific antigen (PSA) expression.
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Roche NimbleGen Webinar Series 
| Date |
Topic |
Speaker |
Download Audio Podcast |
Download Video Podcast |
View Flash Movie |
| May 6, 2010 |
Targeted Re-Sequencing Made Easy: A Core Laboratory’s Experience with NimbleGen SeqCap EZ |
Casey Matthews,
NCI-CGF Scientific Operations Team |
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| Feb 18, 2010 |
Introducing the NimbleGen ChIP-chip and DNA Methylation Multiplex Arrays |
Dr. Ken Lo,
Roche NimbleGen |
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| Jul 29, 2009 |
Introducing the NimbleGen MS 200 Microarray Scanner for Analysis of High-Density DNA Microarrays |
Abigail Farfan,
Roche NimbleGen |
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| Jun 18, 2009 |
Methylation Profiling in Uniparental Tissues Identifies Novel Imprinted Genes |
Dr. Andrew Sharp,
University of Geneva, Switzerland |
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| Apr 9, 2009 |
Introducing the High-Resolution, High-Sensitivity NimbleGen 2.1M DNA Methylation Arrays |
Dr. A. Leonardo Iniguez,
Roche NimbleGen |
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| Jan 15, 2009 |
NimbleGen Sequence Capture Using the HD2 Platform: Exome Capture Made Easy |
Dr. Dan Burgess,
Roche NimbleGen |
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| Nov 6, 2008 |
Introducing NimbleGen HD2 Arrays for High-Resolution ChIP-chip Analysis |
Dr. A. Leonardo Iniguez,
Roche NimbleGen |
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| Apr 3, 2008 |
Sequence Capture Technology: Using Microarrays to Capture Megabases of Sequence or Hundreds of Thousands of Human Exons for Next Generation Sequencing |
Dr. Tom Albert,
Roche NimbleGen |
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| Mar 19, 2008 |
Genomic Analyses of Gene Regulation by Poly(ADP-Ribose) Polymerase-1 and Histone H1 |
Dr. Matt Gamble,
Cornell University |
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| Feb 28, 2008 |
Integrated Epigenomic Analyses of Neuronal MeCP2 Reveal a Role for Long-Range Interaction with Active Genes |
Dr. Janine LaSalle,
UC Davis |
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| Nov 29, 2007 |
Genome Architecture and Genomic Disease: A Targeted Approach to Disease Discovery |
Dr. Heather Mefford
University of Washington |
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| Nov 1, 2007 |
Demonstration of KAP1 Binding to Silenced Chromatin with Genome-wide ChIP-chip Analysis |
Dr. Henriette O'Geen
University of California, Davis |
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| Sep 19, 2007 |
Gene Silencing by Large, Non-coding RNAs: The Regulatory Role of HOTAIR analyzed with ChIP-chip and Tiling Expression Analysis |
Dr. John Rinn, Harvard Medical School |
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| Aug 15, 2007 |
Assaying Gene Expression Using NimbleGen Multiplex Arrays: Taking Advantage of High-Throughput Sample Analysis |
Dr. John Manak, Roche NimbleGen |
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| Jul 18, 2007 |
Genome-Wide Distribution, Sequence Determinant and Evolution of CTCF Binding |
Dr. Tae Hoon Kim, Yale University School of Medicine |
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| May 8, 2007 |
Microarray-Mediated, Whole-Genome Comparison of the Distribution of RNA Polymerase with the Transcript Map in Escherichia coli |
Dr. Joseph Wade, Harvard University |
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| Apr 11, 2007 |
Part I: A novel genomic disorder affecting neurobehavioral development; fine mapping of rearrangements involving complex low copy repeats on chromosome 10q |
Dr. Scott Selleck, The University of Minnesota |
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| Apr 11, 2007 |
Part II: Using Drosophila to model disruptions of PTEN-TSC-TOR signaling on synapse assembly and behavior |
Dr. Scott Selleck, The University of Minnesota |
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| Mar 13, 2007 |
Efficient High-Resolution Deletion Discovery in Caenorhabditis elegans by Array CGH |
Dr. Jason Maydan, UBC, Vancouver, BC, Canada |
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| Mar 6, 2007 |
Using High-Resolution ChIP-chip to Model Chromatin Signatures of Promoters and Enhancers in the Human Genome |
Dr. Nathaniel Henitzman, LICR, UCSD |
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| Feb 15, 2007 |
High-Resolution Mapping of Copy-Number Variants Genome-Wide with Array CGH |
Timothy A. Graubert, M.D. Washington University, St. Louis |
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| Jan 24, 2007 |
Genome-Wide DNA Methylation Analysis with Immunoprecipitation and Tiling DNA Microarrays |
Dr. Daniel Zilberman, Fred Hutchinson Cancer Research Center |
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| Aug 31, 2006 |
The Discovery and Characterization of Genomic Disorders with High-Resolution Array CGH |
Dr. Andrew Sharp, The University of Washington |
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| Aug 8, 2006 |
AHigh-Resolution Method to Identify DNase I Hypersensitive Sites Using Tiled Microarrays |
Dr. Greg Crawford, Duke University |
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| May 16, 2006 |
Genome-Wide Mapping of Transcriptional Regulatory Elements in Mammalian Cells |
Dr. Bing Ren, UCSD |
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| Feb 6, 2006 |
Identifying Antibiotic Resistance and Other Adaptive Mutations in Helicobacter pylori using NimbleGen CGS Technology |
Dr. Douglas Berg, Washington University |
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Roche NimbleGen Workshops
| Date |
Venue |
Topic |
Speaker |
Download Audio Podcast |
Download Video Podcast |
View Flash Movie |
| May 25, 2009 |
ESHG 2009 |
High Resolution Array CGH in Chronic Myeloid Leukemia |
Dr. Alistair Reid
Imperial College of London |
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| Developing an Imprinting Map of the Human Genome |
Dr. Andrew Sharp
University of Geneva |
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| Jan 13, 2009 |
PAG 2009 |
Empirical Annotation of Genomes by Differential Expression Analysis of Transcriptionally Active Regions |
Dr. John Colbourne
Indiana University |
Audio and Video Files Available - Fall 2009 |
| Whole Genome Analysis of DNA Copy Number Variation in Dogs |
Dr. Carlos E. Alvarez
Ohio State University |
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| Discovery of Human Genetic Variations with Sequence Capture Technology |
Dr. Nathan M. Springer
University of Minnesota |
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| Nov 12, 2008 |
ASHG 2008 |
Copy Number Variation in Low Copy Repeat-Rich Regions of the Genome: How Much is There and What Does it Take to Measure It? |
Dr. Scott Selleck
University of Minnesota |
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| Distinct Chromatin Modifications at Enhancers Correlate with Cell Type-Specific Gene Expression in the Human Genome |
Dr. David Hawkins
University of California - San Diego |
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| Discovery of Human Genetic Variations with Sequence Capture Technology |
Matthew Bainbridge
MS Student
Baylor College of Medicine |
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| Jun 2, 2007 |
ESHG 2008 |
High-Throughput Approaches for DNA Methylation Profiling |
Dr. Stephan Beck
University College London |
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| Sequence Capture for Medical Sequencing |
Dr. Daniel Turner
Wellcome Trust Sanger Institute |
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| Jan 15, 2008 |
PAG 2008 |
Genome-Scale Targeted Sequencing and Expression Analysis in Duplicated Genomes |
Full Program |
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Direct Genomic Selection by Microarray Hybridization for
High-Throughput Sequencing |
Dr. Xinmin Zhang,
Roche NimbleGen |
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| Insights into the Evolutionary Significance of Whole Genome Duplications Provided by Populus Expression Arrays |
Dr. Steve DiFazio,
West Virginia University |
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| Duplicate gene expression evolution in cotton |
Lex Flagel,
PhD Student
Iowa State University |
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| Oct 25, 2007 |
ASHG 2007 |
Genome-wide scan of copy number variation in autism |
Dr. Jonathan Sebat
Cold Spring Harbor Laboratory |
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| Epigenetic Regulation of the HOX Locus by Non-coding RNAs |
Dr. John Rinn, Harvard Medical School |
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| Jun 18, 2007 |
ESHG 2007 |
Human Genetics and Epigenetics:
High-Resolution Discovery with Array CGH, DNA Methylation, and ChIP-chip |
Full Program |
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| Towards a comprehensive map of copy number variation in the human genome |
Dr. Matthew Hurles
The Wellcome Trust Sanger Institute, Cambridge |
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Mapping promoter DNA
methylation in mammalian
genomes using oligonucleotide
arrays |
Dr. Michaël Weber
Friedrich Miescher Institute for Biomedical Research, Basel |
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| Combinatorial Effects of Four Histone Modifications in Transcription and Differentiation |
Dr. Silke Sperling
Max Planck Institute for Molecular Genetics, Berlin |
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| Oct 10, 2006 |
ASHG 2006 |
Human Genetics and Epigenetics: High-Resolution Discovery with Array CGH, DNA Methylation, and ChIP-chip |
Full Program |
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| The Discovery and Characterization of Genomic Disorders with High-Resolution Array CGH |
Dr. Andrew Sharp, The University of Washington |
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| Cytosine Methylation Dynamics in Development and Disease |
Dr. John Greally, Albert Einstein College of Medicine |
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| ChIP-Chip'ing Away at the Functions of Proteins Mutated in Genetic Syndromes |
Dr. Peter C. Scacheri, Case Western Reserve University |
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Learn More About Our Video and Audio Formats
Listening to the Audio Podcasts
The audio files are posted in mp3 format and are compatible with any mp3 player, including all iPod models, Zune, etc. If you are running iTunes, you have the option of subscribing to the Roche NimbleGen Webinar Series or downloading the podcast files individually and opening them with iTunes. You can listen to the files on your desktop with iTunes, Winamp, Windows Media Player, etc. or transfer them to your iPod for portability.
Viewing the Video Podcasts
Viewing the video Podcasts requires Apple iTunes and a fifth generation video-capable iPod or an Apple iPhone. You have the option of subscribing to the NimbleGen Systems Webinar Series through iTunes or downloading the podcast files individually and opening them with iTunes. You can view the files on your desktop with iTunes or transfer them to your iPod for portability. They are posted in an MPEG-4 video file format.
Viewing the Movies
Viewing the video of the Roche NimbleGen webinars and workshops requires the Adobe Flash Plugin.
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